In vitro phagocytosis model of endometrial cancer cells by macrophages via live cell application
10.19405/j.cnki.issn1000-1492.2022.09.023
- Author:
Xuewen Tan
1
;
Weile Chen
1
;
Yizhao Chen
1
;
Yilong Fang
1
;
Haifeng Jiang
1
;
Zhen Xu
1
;
Jiajie Tu
1
;
Wei Wei
1
Author Information
1. Institute of Clinical Pharmacology,Anhui Medical University,Key Lab ofAnti⁃inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti⁃inflammatory and Immune Medicine ,Hefei 230032
- Publication Type:Journal Article
- Keywords:
live cell application;
macrophages;
Ishikawa cells;
phagocytosis;
cytotoxic T cells
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1465-1465-1469
- CountryChina
- Language:Chinese
-
Abstract:
Objective :The live cell application was used to observe the process of phagocytosis of endometrial cancer cells by macrophages, and flow cytometry was used to detect the effects of macrophages engulfing tumor cells on activation of cytotoxic T cell.
Methods : Ishikawa cells and THP1-induced macrophages were labeled with CFSE fluorescent probe and CD11 b respectively, and then mixed and seeded on a glass imaging dish.The live cell application was performed to record the phagocytosis of Ishikawa cells by macrophages within 120 minutes.Flow cytometry was used to detect the effect of macrophages engulfing tumor cells on activation of cytotoxic T cell.
Results :The green fluorescence of Ishikawa cells was taken up by macrophages after the co-cultured two types of cells were in contact with each other, and macrophages were able to engulf multiple Ishikawa cells continuously.Macrophages that engulfed Ishikawa cells could induce activation of cytotoxic T cell.
Conclusion: The live cell application was successfully conducted to construct an in vitro model of phagocytosis of tumor cells by macrophages, which provided a feasible experimental method for detecting the dual killing process of macrophages and T cells on tumor cells.
- Full text:2024122715053759460利用活细胞工作站构建巨噬细...噬子宫内膜癌细胞的体外模型_檀学文.pdf
