Effect and mechanism of cathepsin S on proliferation, migration and invasion of osteosarcoma cells
10.19405/j.cnki.issn1000-1492.2022.09.022
- Author:
Hairu Ji
1
;
Lingwei Kong
2
;
Sheng Cao
2
;
Jiaxing Lü
1
;
Jiaxin Li
1
;
Chunyu Liu
1
;
Yu Jin
2
Author Information
1. Dept of Pathology, Chengde Medical College , Chengde 067000
2. Dept of Trauma Surgery, Hospital of Chengde Medical College , Chengde 067000
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
cathepsin S;
small interfering RNA;
proliferation;
Wnt/β⁃catenin
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1459-1465
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of cathepsin S(Cat S) on proliferation, migration and invasion of osteosarcoma cells and its potential regulatory mechanism.
Methods:Normal osteoblasts(hFOB) and osteosarcoma cells(SAOS2 and MG63) were selected as the subjects of this study.Cat S small interfering(si) RNA(si-Cat S) and negative control sequence(si-NC) were transfected into SAOS2 and MG63 cells to modulate the expression of Cat S in osteosarcoma cells.The experimental cells were randomly divided into four groups: hFOB group(hFOB cells),Control group(untransfected SAOS2 or MG63 cells),si-NC group(SAOS2 or MG63 cells transfected with si-NC) and si-Cat S group(SAOS2 or MG63 cells transfected with si-Cat S).The expression of Cat S in SAOS2 and MG63 cells was detected by RT-PCR and Western blot.Effect of Cat S knockdown on the proliferation of SAOS2 and MG63 cells was assessed by CCK-8 and clone formation assays.And effects of Cat S knockdown on the migration and invasion of SAOS2 and MG63 cells were determined by wound-healing and Transwell assays, respectively.Western blot assay was performed to measure the effects of SAOS2 knockdown on the expressions of apoptosis-related proteins(Bcl-2,Bax and Caspase-3) and Wnt/β-catenin pathway related proteins(LRP5,β-catenin, C-myc and Cyclin D1) in SAOS2 cells.
Results:Compared with hFOB group, the expression of Cat S in SAOS2 group and MG63 group was upregulated(P<0.001).In addition, compared with si-NC group, the proliferation, migration and invasion of cells in si-Cat S group were reduced(P<0. 001). Results of Western blot showed that compared with si-NC group,the expression of Bcl-2 in si-Cat S group was downregulated,while the expression of Bax and Caspase-3 were upregulated(P<0. 001). Meanwhile,compared with si-NC group,the expression of LRP5,β-catenin,C-myc and Cyclin D1 in si-Cat S group was downregulated(P<0. 001).
Conclusion:Cat S siRNA knockdown can inhibit the proliferation,migration and invasion of osteosarcoma cells and induce apoptosis by regulating Wnt/β-catenin pathway,indicating that Cat S may be one of the potential targets for the treatment of osteosarcoma.
- Full text:2024122714585228524组织蛋白酶S对人骨肉瘤细胞...、迁移、侵袭的影响及其机制_纪海茹.pdf
