Disulfiram synergizes with oxaliplatin to induce ferroptosis in gastric cancer cells
10.19405/j.cnki.issn1000-1492.2022.09.021
- Author:
Xin Yu
1
;
Yi Liu
1
;
Bo Zhou
1
;
Xiandong Cao
1
Author Information
1. Dept of Gastrointestinal Surgery, The First Afiliated Hospital of Anhui Medical University,Hefei 230022
- Publication Type:Journal Article
- Keywords:
gastric cancer;
disulfiram;
oxaliplatin;
synergizes;
ferroptosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1453-1458
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the synergistic inhibitory effect of disulfiram (DSF) combined with Oxaliplatin (Oxa) on gastric cancer cells BGC⁃823.
Methods : Cell viability was assessed with CCK⁃8 assay. CompuSyn software was used for fitting the Fraction⁃Effect curve to evaluate the combined effect of the two agents. AnnexinV/ PI staining detected apoptosis rate of BGC⁃823 by flow cytometry. Pyridine iodide (PI) staining detected the rate of dead cells by flow cytometry. BODIPY 581/591 C11 dye (Invitrogen) staining detected lipid peroxidative free radicals (LipROS) level by flow cytometry. qRT⁃PCR detected the mRNA levels of ferroptosis⁃related markers PTGS2 , GPX4 and SLC7A11 .
Results :DSF and Oxa alone or in combination could inhibit the viability of BGC⁃823 cells in a time⁃ and dose⁃dependent manner, and the combination of the two drugs could exert a synergistic effect. DSF syn⁃
ergized with Oxa to induce apoptosis and ferroptosis in gastric cancer cells. The combination of DSF and Oxa could induce significantly increased LipROS level , resulting in a significant increase in the ferroptosis marker PTGS2mRNA level and a significant decrease in the ferroptotic markers GPX4 and SLC7A11 mRNA levels.
Conclusion :Disulfiram synergizes with Oxaliplatin to inhibit the cell viability , induce BGC⁃823 cell apoptosis and ferroptosis.
- Full text:2024122711243118825双硫仑协同奥沙利铂诱导胃癌细胞铁死亡_喻鑫.pdf
