The effect of hydroquinone on autophagy of L-02 hepatocytes
10.19405/j.cnki.issn1000-1492.2022.09.016
- Author:
Zuobing Zheng
1
,
2
;
Wei Zhang
3
;
Liang Xiong
3
;
Tingting Liu
3
;
Yangna Wu
3
;
Jiahui Yang
3
;
Gonghua Hu
1
,
2
,
4
Author Information
1. School of Public Health ,Nanchang University,Nanchang 330006
2. Jiangxi Provincial Key Laboratory of Preventive Medicine ,Nanchang 330006
3. School of Public Health and Health Management , Gannan Medical University, Ganzhou 341000
4. School of Public Health and Health Management , Gannan Medical University, Ganzhou 341000
- Publication Type:Journal Article
- Keywords:
hydroquinone;
hepatocyte;
autophagy
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1425-1430
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the effect of hydroquinone on autophagy of human L-02 hepatocytes.
Methods : L-02 cells were treated with different concentrations of hydroquinone(0,10,20,40 and 80 μmol/L) for 24 hours, the formation of autophagosomes was observed by transmission electron microscope;autophagosome formation was traced by mCheery-GFP-LC3B fusion protein. Western blot experiment was used to detect the expression of autophagy marker proteins LC3,Beclin-1,P62;immunofluorescence experiment was used to detect the subcellular localization and expression of autophagy proteins LC3,Beclin-1,P62.
Results :Under the transmission electron microscope,it was observed that the autophagosomes and lysosomes of L-02 cells increased after the action of hydroquinone. Under the fluorescence microscope,the number of GFP-LC3B punctate yellow spots increased with the increase of hydroquinone dose,while the red flake spots decreased. Western blot results showed that hydroquinone increased the ratio of LC3Ⅱ/LC3Ⅰ protein in L-02 cells,and the difference between treatment groups and the control group was statistically significant(P<0. 05). Hydroquinone had no significant effect on Beclin-1 and P62 protein expression in L-02 cells(P>0. 05). The results of immunofluorescence experiments showed that LC3 and Beclin-1 were both expressed in the nucleus and cytoplasm of L-02 cells,hydroquinone had no effect on the subcellular localization of LC3 and Beclin-1 in L-02 cells,while 40 and 80 μmol/L hydroquinone could induce the translocation of P62 in L-02 cells from the cytoplasm to the nucleus.
Conclusion:Hydroquinone induces the increase of autophagosomes in L-02 cells,which may be related to the obstacles to autophagosome clearance.
- Full text:2024122617475350651氢醌对L-02肝细胞自噬的影响_郑作兵.pdf
