Chrysin inhibits proliferation and promotes apoptosis of ovarian cancer cells by inducing autophagy
10.19405/j.cnki.issn1000-1492.2022.04.002
- Author:
Yuchuan Shi
1
;
Yu He
2
;
Yang Yang
1
;
Yijun Fan
1
;
Fang Yang
1
;
Lei Zhan
1
;
Bing Wei
1
Author Information
1. Dept of Obstetrics and Gynecology，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601
2. Dept of Pharmacy，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601
- Publication Type:Journal Article
- Keywords:
SKOV3 cells;
autophagy;
proliferation;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(4):510-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of chrysin and chloroquine(CQ) on the proliferation and apoptosis of ovarian cancer cells.
Methods:Ovarian cancer specimens and normal ovarian specimens were selected after surgical resection and immunohistochemical experimental methods was used to detect the expression level of autophagy-related protein LC3 in ovarian cancer and normal ovarian tissues. The CCK-8 method was used to detect the proliferation of ovarian cancer cell SKOV3. Western blot and immunofluorescence were used to detect the expression of LC3 in SKOV3 cells. An electron microscope was used to detect the number of autophagosomes in SKOV3 cells. Flow cytometry was used to detect SKOV3 cell apoptosis.
Results:The expression level of LC3 in ovarian cancer was higher than that in normal ovarian tissues. SKOV3 cells were treated with chrysin at concentrations of 0, 20, 40, 60, 80, 100 μmol/L for 24 hours. As the concentration increased, cell proliferation decreased significantly. Compared with the control group, 40 μmol/L, chrysin significantly up-regulated the expression of LC3 after 24 hours(P<0.05). Compared with the control group, after 24 hours of SKOV3 cells treated with chrysanthemum, more autophagosomes were observed under transmission electron microscope(P<0.05), but no autophagosome was observed in the control group. When chrysin and chloroquine were used in combination for 24 hours, the expression of LC3 protein in the combined treatment group was higher than that in the chrysin and CQ treatment group(P<0.05). Chrysin could obviously induce apoptosis of SKOV3 cells, and the group of Chrysin+CQ showed the highest proportion of apoptosis(P<0.05).
Conclusion:Chrysin can induce autophagy to inhibit the proliferation of ovarian cancer cells and promote apoptosis.
- Full text:2024122615111617139白杨素联合氯喹抑制卵巢癌细胞增殖促进凋亡的研究_史于传.pdf
