Exploring breast cancer microenvironment and chemoresistance based on 3D in vitro microtumour models
10.19405/j.cnki.issn1000-1492.2024.11.016
- Author:
Juanru Wang
1
;
Qiaozhii Song
2
;
Xiaoli Liu
1
,
3
;
Zhengsheng Wu
1
,
3
Author Information
1. Dept of Pathology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
2. The First Clinical College , Anhui Medical University , Hefei 230032
3. Dept of Pathology , Anhui Medical University , Hefei 230032Dept of Pathology , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
3D culture;
co-culture;
core-shell microcapsules;
tumor microenvironment;
chemoresistance
- From:
Acta Universitatis Medicinalis Anhui
2024;59(11):2004-2012
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To simulate the tumor microenvironment though the 3D microtumor model which was constructed using droplet microfluidics.To explore its feasibility as a model for in vitro breast cancer research through3D microtumour fabrication,characterisation and sensitivity testing to chemotherapeutic drugs.
Methods:Breast cancer cells were encapsulated with hydrogel shells in collagen-rich microencapsulated cores to obtain breast cancer microtumours in vitro;breast cancer microtumours were co-cultured with 3D microencapsulated endothelial cells by Transwell system.The structure and growth characteristics of the microtumours were directly observed by microscopy;the CCK-8 assay was used to detect the proliferation of the cells under different culture models and the drug sensitivity to doxorubicin;flow cytometry was used to compare the differences in apoptosis during the proliferation process;and the differences in the migratory and invasive abilities of the cells were assessed by scratch assay and Transwell assay;the expression of epithelial-mesenchymal transition-related proteins was detected by Western blot.
Results:Breast cancer cells grew well in hydrogel nucleus-shell microcapsules;cell proliferation assays showed that 3D culture and 3D co-culture cells proliferated at a significantly lower rate than 2D culture;3D culture and 3D co-culture cells had enhanced migration and invasion ability and showed higher expression of EMT-related proteins compared to 2D culture;3D culture and 3D co-culture cells were significantly less sensitive to chemotherapeutic drugs compared to 2D culture.The sensitivity of 3D and 3D co-cultured cells to chemotherapeutic drugs was significantly reduced compared to 2D culture.
Conclusion:3D cultures show similar morphology and biology to in vivo tumours and are more resistant to chemotherapeutic agents.
- Full text:2024122614033147776基于3D微肿瘤模型探讨乳腺癌微环境及耐药性_王娟如.pdf
