Effects of long-term hyperglycemia on renal pathology of type 1 diabetic mellitus cynomolgus monkeys
10.19405/j.cnki.issn1000-1492.2024.11.015
- Author:
Xinyu Kuang
1
,
2
;
Peiwen Jiang
1
,
2
;
Dongjun Wu
1
;
Chunlin Zou
1
,
3
;
Qiong Song
1
,
3
Author Information
1. School of Preclinical Medicine ,Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021
2. Center for Translational Medicine ,Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021Key Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry ofEducation ,Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021
3. Center for Translational Medicine ,Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021Key Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry ofEducation ,Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021Institute of Neuroscience and Guangxi Key Laboratory of Brain Science ,Institute of Neuroscience and Guangxi Key Laboratory of Brain Science ,Institute of Neuroscience and Guangxi Key Laboratory of Brain Science ,Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry , Guangxi Key Laboratory of Regenerative Medicine , Guangxi Medical University , Nanning 530021
- Publication Type:Journal Article
- Keywords:
streptozotocin;
type 1 diabetic mellitus;
cynomolgus monkeys;
kidney;
podocyte;
pathology
- From:
Acta Universitatis Medicinalis Anhui
2024;59(11):1998-2003
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of long-term hyperglycemia on renal pathology of type 1 diabetic mellitus(T1DM) cynomolgus monkeys by establishing streptozotocin(STZ)-induced T1DM.
Methods:Eight 4-year-old male cynomolgus monkeys were randomly divided into control and model groups. Four cynomolgus monkeys were used in the control group, and four cynomolgus monkeys were injected with streptozotocin to create a T1DM model. Hematoxylin-eosin(HE) staining and periodic acid-Schiff(PAS) staining were used to observe renal pathological changes.The histological characteristics and changes were observed under transmission electron microscope. Morphometric measurements were used to analyze the glomerular area, the thickness of the glomerular basement membrane, the proportion of glomerular podocyte foot processes and the average area of foot processes.
Results:In comparison to the control group, the model group showed pathological changes in the kidneys, including increased glomerular area(P<0.01), thickened basement membrane, capillary loop compression, disordered arrangement of endothelial cell fenestrations, proliferation of mesangial cells, mesangial expansion, accumulation of mesangial matrix glycoproteins, a decrease in the number of foot processes(P<0.001), a decrease in the average size of the foot processes(P<0.05), widened podocyte slit diaphragm, and an increase in the number of fused foot processes(P<0.001).
Conclusion:Cynomolgus monkeys with T1DM suffer from renal pathological changes due to long-term hyperglycemia.
- Full text:2024122613584839652长期高血糖对1型糖尿病食蟹猴模型肾脏病理学的影响_况昕宇.pdf
