M1 polarization of macrophage induced by STING signaling promotes T cell immune response
- Author:
Jianfei Li
1
;
Zhi Duan
1
;
Qian Liu
1
;
Qiyin Zong
1
;
Wanlu Duan
1
;
Futing Liu
1
;
Hao Zhang
1
;
Qiang Zhou
1
;
Qin Wang
1
Author Information
- Publication Type:Journal Article
- Keywords: macrophage; STING signaling; polarization of macrophage; T cell immune response; CD4 + T cells; CD8 + T cells
- From: Acta Universitatis Medicinalis Anhui 2024;59(11):1974-1981
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of activation of the stimulator of interferon genes(STING) pathway on macrophage polarization function and its role in T-cell response.
Methods:Mouse macrophage RAW264.7 cells were used.STING signaling related proteins in RAW264.7 macrophage treated with STING agonist diABZI were analyzed by Western blot,including TANK-binding kinase-1(TBK1),interferon regulatory factor-3(IRF3),STING,p-TBK1,p-IRF3,p-STING.The polarization of macrophage RAW264.7 cells treated with diABZI was analyzed by flow cytometry.Co-culture of diABZI-treated RAW264.7 macrophage and T cells was applied to evaluate the change of T cell response.
Results:STING signaling related proteins were upregulated in macrophage RAW264.7 cells treated with diABZI for 3 hours.The expression of CD86 was upregulated on the surface of macrophages after 12 hours of diABZI treatment,and the CD86/CD206 ratio was elevated,which presented the M1 polarization phenotype.When coculturing diABZI-treated macrophage RAW264.7 cells with T cells,the cytokine secretion ability of T cells including CD4+T and CD8+T cells was enhanced and the expression of CD107a in CD8+T cells was upregulated.
Conclusion:STING signaling induces M1 polarization of macrophages which enhance the function of T cells,especially CD8+T cell immune response. - Full text:2024122613420020063STING通路的活化诱导巨...细胞极化促进T细胞免疫应答_李健飞.pdf
