Expression of enolase and laminin in the vagina of bacterial vaginosis of mice
10.19405/j.cnki.issn1000-1492.2024.11.008
- Author:
Rui Zhang
1
;
Yiqing Wu
2
,
3
,
4
,
5
;
Haixu Yin
1
;
Chang Li
1
;
Aixin Ma
1
;
Junzhu Wang
6
;
Huihui Bai
7
;
Zhaohui Liu
7
;
Qinping Liao
8
Author Information
1. Dept of Obestetrics and Gynecology, Peking University First Hospital , Beijing 100034
2. Northwest Women &prime
3. s and Childern &prime
4. s Hospital , Xi &prime
5. an 710061
6. Big Data and Public Policy Laboratory , School of Government , Peking University , Beijing 100091
7. Dept of Obestetrics and Gynecology, Beijing Obstetrics and Gynecology Hospital , Beijing 100026
8. Dept of Obestetrics and Gynecology, Beijing Tsinghua Changgung Hospital , Beijing 102218
- Publication Type:Journal Article
- Keywords:
animal mode;
bacterial vaginosis;
enolase;
laminin;
Gardnerella vaginalis;
pathogenesis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(11):1939-1943
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences in enolase and laminin levels in vaginal epithelial tissues between mice successfully infected withGardnerellaand mice not infected with Gardnerella, providing information for further exploration of the correlation between enolase and laminin levels and the incidence of bacterial vaginosis.
Methods:Gardnerella strains isolated, purified, and identified from vaginal secretions of patients with bacterial vaginosis were used to infect the vagina of mice and establish a mouse model of bacterial vaginosis. Successful and failed mice was defined as successful and failed groups, respectively. Differential expression of enolase and laminin in the vaginal epithelial tissue of two groups of mice was detected by Western blot. Modeling success rate was statistically analyzed, and the expression differences of enolase and laminin was compared between two groups.
Results:One strain of Gardnerella vaginalis infected 10 SPF grade KM mice, 7 mice met the diagnostic criteria for bacterial vaginosis, and 3 mice failed to model, with a success rate of 70%. Western blot was used to detect protein expression levels, and the levels of laminin and enolase in the successfully modeled mouse vaginal epithelial tissue were significantly higher than those in the failed modeling group, with statistical differences between the two groups(P<0.05).
Conclusion:Enolase and laminin may be involved in the occurrence of bacterial vaginosis, however, further research is needed to determine the mechanisms through which they trigger the occurrence and development of the disease.
- Full text:2024122613073088956烯醇化酶和层黏连蛋白在细菌...阴道病模型小鼠阴道中的表达_张瑞.pdf
