Pancreatic stellate cells promote the PIK3C2A expression and growth of pancreatic cancer cells through paracrine effects
10.19405/j.cnki.issn1000-1492.2024.11.005
- Author:
Zhan Yue
1
;
Kemiao Zhen
2
;
Haozhe Cui
2
;
Wantao Ying
3
Author Information
1. School of Basic Medical Sciences , Anhui Medical University , Hefei 230032
2. State Key Laboratory of Medical Proteomics , Beijing Proteome Research Center , National Center for Protein Sciences (Beijing) , Beijing Institute of Lifeomics , Beijing 102206
3. School of Basic Medical Sciences , Anhui Medical University , Hefei 230032；State Key Laboratory of Medical Proteomics , Beijing Proteome Research Center , National Center for Protein Sciences (Beijing) , Beijing Institute of Lifeomics , Beijing 102206
- Publication Type:Journal Article
- Keywords:
pancreatic stellate cell;
conditioned medium;
indirect co-culture;
proteomics
- From:
Acta Universitatis Medicinalis Anhui
2024;59(11):1919-1925
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect and regulatory mechanism of secreted proteins from PSC on pancreatic ductal adenocarcinoma cells(PANC-1).
Methods:Conditioned medium(CM) from pancreatic stellate cells(PSC) was collected through an indirect co-culture method, and PANC-1 cells were cultured separately with CM for 0, 2, and 24 h. The proliferation phenotype of PANC-1 cells under different stimulation periods was detected using the CCK-8 assay. Proteomic analysis was performed to analyze the changes in protein levels of PANC-1 cells, and the most significant protein changes were validated using Western blot.
Results:Compared with the control group, the proliferation rate of PANC-1 cells increased after being stimulated by PSC derived CM; The results of proteomic analysis showed that the protein expression of metabolic pathways in PANC-1 cells increased continuously after being cultured in PSC CM for 0, 2, and 24 h. Western blot analysis confirmed an increasing trend of PIK3C2A in PANC-1 cells, indicating that the CM from PSC might promote the proliferation of PANC-1 cells by upregulating the expression of PIK3C2A.
Conclusion:The CM of PSC may promote the proliferation of PANC-1 cells by upregulating the expression of PIK3C2A, which improves the understanding of the mechanism of interaction between PSCs and pancreatic cancer cells in the tumor microenvironment.
- Full text:2024122612502911754胰腺星状细胞通过旁分泌效应...的PIK3C2A表达及生长_岳展.pdf
