Curcumin attenuates IL-1β-induced chondrocyte damage by modulating the DUSP1/p38 MAPK pathway
10.19405/j.cnki.issn1000-1492.2024.11.003
- Author:
Fei Song
1
,
2
;
Xuefei Fan
3
;
Nannan Liu
3
;
Suhuan Chen
3
;
Min Jiang
2
;
Guangyi Chen
4
;
Wuqi Chen
4
;
Xiaoyu Chen
3
;
Jian Zhou
5
Author Information
1. Dept of Orthopaedics , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
2. Dept of Orthopaedics , No. 901 Hospital , PLA , Hefei 230031
3. Dept of Histology and Embryology ,Anhui Medical University , Hefei 230022
4. Dept of Clinical Medicine , Anhui Medical University , Hefei 230022
5. Dept of Orthopaedics , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
curcumin;
osteoarthritis;
cartilage;
DUSP1;
p38 MAPK
- From:
Acta Universitatis Medicinalis Anhui
2024;59(11):1903-1910
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of curcumin(Cur) on IL-1β-induced cartilage damage and to study the relationship between the regulatory mechanisms of the DUSP1/p38 MAPK signalling pathway in the above process.
Methods:Chondrocytes(C28/I2) and postoperative primary chondrocytes from osteoarthritis patients were divided into control and experimental groups, and the experimental group was treated with different concentrations of Cur(0, 10, 20, 40, 60, 80 μmol/L) after applying the inflammatory induction treatment with IL-1β(10 μg/L). The cell proliferation inhibition rate was determined by cell viability assay(CCK-8), the apoptosis rate was detected by flow cytometry assay. Real-time fluorescence quantitative PCR(qRT-PCR), Western blot, and immunofluorescence assay were used to detect type II collagen α1 chain(Collagen Ⅱ), matrix metallopeptidase 13(MMP13), interleukin-1β(IL-1β), BCL2-related X protein(Bax), B lymphocytoma-2(Bcl-2), dual-specificity phosphatase 1(DUSP1), p38 mitogen-activated protein kinase(p38), and phosphorylated p38 mitogen-activated protein kinase(p-p38) RNA and protein expression levels. The role of the DUSP1/p38 MAPK axis in the inhibition of chondrocyte oxidative stress, apoptosis and inflammation by Cur was further validated using DUSP1 interfering RNA and p38 MAPK pathway inhibitor(SB).
Results:Cur significantly inhibited the IL-1β-induced decrease in chondrocyte viability and significantly reduced the levels of oxidative stress, apoptosis, and inflammation in chondrocytes; Cur inhibited the expression of MMP13, IL-1β, Bax, and p-p38 proteins, while the expression of Collagen II, Bcl-2, and DUSP1 proteins significantly increased; IL-1β and interfering RNA silencing DUSP1 activated the p38 pathway, while Cur inhibited the activation of the p38 pathway; the use of p38 MAPK pathway inhibitors reduced cellular inflammation.
Conclusion:Cur attenuates IL-1β-induced oxidative stress, apoptosis and inflammation in chondrocytes by promoting the expression of DUSP1 protein and inhibiting the activation of p38 MAPK pathway.
- Full text:2024122612430833741姜黄素通过调控DUSP1_...L-1β诱导的软骨细胞损伤_宋飞.pdf
