The absence of GPR108 results in decreased inflammatory response in lipopolysaccharide-induced sepsis mice
10.19405/j.cnki.issn1000-1492.2024.11.002
- Author:
Yintao Zhang
1
;
Ping Yang
1
;
Dandan Zang
2
;
Zhenzhen Tu
3
;
Ruyue Xu
1
;
Haisheng Zhou
4
Author Information
1. Dept of Biochemistry ,Anhui Medical University , Hefei 230032
2. Center Scientific Research ,Anhui Medical University , Hefei 230032
3. Institute of Clinical Immunology , Anhui Medical University , Hefei 230032
4. Dept of Biochemistry ,Anhui Medical University , Hefei 230032；Center Scientific Research ,Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
sepsis;
G protein-coupled receptor 108;
macrophage;
lipolyaccharide;
interleukin 6
- From:
Acta Universitatis Medicinalis Anhui
2024;59(11):1896-1902
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of G protein-coupled receptor 108(GPR108) gene knockout on systemic inflammation in lipopolysaccharide(LPS)-induced sepsis mice.
Methods:Male C57BL/6 mice and GPR108 gene knockout mice were randomly divided into 4 groups: WT group, WT-LPS group, KO group, KO-LPS group. The physiological characteristics of mice in different groups were observed, and the morphological changes of liver and lung tissues were observed. Macrophages were extracted from bone marrow and subjected to flow cytometry to detect their M1 polarization status. The expression levels of IL-6 in liver and lung tissues, macrophages, and serum were also measured.
Results:KO-LPS group mice showed significant liver and lung tissue damage, with a significantly greater number of bone marrow-derived macrophages polarizing towards M1 in the KO-LPS group compared to the WT-LPS group. Additionally, at the tissue, cellular, and serum levels, the expression of IL-6 in the KO-LPS group mice was significantly higher than that in the WT-LPS group mice(P<0.05).
Conclusion:During the systemic inflammatory infection induced by LPS in mice, the lack of GPR108 exacerbates the systemic inflammatory response. GPR108 has an inhibitory effect on the inflammatory response in mice with LPS-induced sepsis.
- Full text:2024122612361505834GPR108缺失对脂多糖诱发脓毒症小鼠的炎症反应_张银涛.pdf
