Development of a Quantitative Chromatographic Fingerprint Analysis Method for Sugar Components of Yuanhu Zhitong Oral Liquid Using HPLC-CAD

Jing LAN; Jiale XIE; Zhiming CAO; Jianli GUAN; Yi WANG; Xingchu GONG

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Development of a Quantitative Chromatographic Fingerprint Analysis Method for Sugar Components of Yuanhu Zhitong Oral Liquid Using HPLC-CAD

VernacularTitle:基于HPLC-CAD的元胡止痛口服液糖类成分定量指纹图谱研究
Author: Jing LAN ¹ ; Jiale XIE ¹ ; Zhiming CAO ² ; Jianli GUAN ² ; Yi WANG ³ ; Xingchu GONG ³
Author Information

1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China；Innovation Institute for Artificial Intelligence in Medicine, Zhejiang University, Hangzhou 310018, China
2. Henan Fusen Pharmaceutical Co., Ltd., Nanyang 474450, China
3. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China；Innovation Institute for Artificial Intelligence in Medicine, Zhejiang University, Hangzhou 310018, China；State Key Laboratory of Chinese Medicine Modernization, Zhejiang University, Hangzhou 310058, China
Publication Type:Journal Article
Keywords: HPLC-CAD； Yuanhu Zhitong oral liquid；sugar ；quantitative chromatographic fingerprint
From: Chinese Journal of Modern Applied Pharmacy 2024;41(12):1694-1698
CountryChina
Language:Chinese
Abstract: OBJECTIVE :To establish a quantitative fingerprint analysis method for sugar components in Yuanhu Zhitong oral liquid using high performance liquid chromatography-charged aerosol detection(HPLC-CAD). METHODS Chromatographic column was NH2P-50 4E(4.6 mm×250 mm, 5 μm) column. Water(A) and acetonitrile(B) were used as the mobile phase in the gradient elute mode. The column temperature was 30 ℃. The injection volume was 10 μL. The flow rate was 0.6 mL·min−1. The evaporation temperature of CAD was 35 ℃. The acquisition frequency was 10 Hz. The power function value was 1.0. RESULTS The linear relationship of the quantitative component was good within the quantitative range, with R2>0.999. The relative standard deviations(RSDs) of instrument precision, intermediate precision and method repeatability were all <3%. The test solution was stable within 24 h. The average recoveries at low, medium and high concentration levels ranged 97.15%−101.13%. There were 5 common peaks in the fingerprint. The RSDs of instrument precision, method repeatability and sample stability were all <4%. CONCLUSION The established analytical method is stable, accurate and reproducible. It can be used to detect sugar excipients in the preparations.