Effect of Saikosaponins and Paeoniflorin on Neurotransmitters in the Hippocampal Brain Region of Rats with Premenstrual Dysphoric Disorder Model of Liver Qi Depression Using UHPLC-MS/MS Target Metabolomics
10.13748/j.cnki.issn1007-7693.20224251
- VernacularTitle:利用UHPLC-MS/MS靶标代谢组学研究柴芍皂苷对经前烦躁障碍症肝气郁证模型大鼠海马脑区神经递质的影响
- Author:
Xianliang SONG
1
;
Xiangjun WANG
2
;
Xiaowen XIA
2
;
Yashuang REN
1
;
Dedi GUO
1
;
Chunhong SONG
2
Author Information
1. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
2. Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, China
- Publication Type:Journal Article
- Keywords:
saikosaponins and paeoniflorin；premenstrual dysphoric disorder ；liver Qi depression；neurotransmitters
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(12):1655-1662
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To study the therapeutic effect of saikosaponins and paeoniflorin on rats with premenstrual dysphoric disorder(PMDD) model of liver Qi depression and the effect on neurotransmitters in hippocampal brain area.
METHODS
The PMDD rat model of liver Qi depression was prepared by the time-chosen chronic restraint stress method, and the depressive symptoms of rats were evaluated by the intervention of saikosaponins and paeoniflorin, the open field experiment and forced swimming experiment, and the changes of neurotransmitter levels in the hippocampal brain region of rats were detected by UHPLC-MS/MS target metabolomics.
RESULTS
The levels of 5-HT, Glu, GABA and NE in the hippocampal brain area of PMDD rats were significantly increased, and the levels of 5-HT, Glu, GABA, NE, DA and E were not statistically significant from those of the normal group after the intervention of saikosaponins and paeoniflorin.
CONCLUSION
Saikosaponins and paeoniflorin alleviates depression in rats with PMDD by regulating neurotransmitter levels, and it may be the pathway for the clinical treatment of PMDD with liver qi depression in the formulations of Bupleuri Radix and Paeoniae Radix Alba.
