Effects of polystyrene microplastics on the growth,biofilm formation and virulence of Klebsiella pneumoniae
10.19405/j.cnki.issn1000-1492.2024.10.020
- VernacularTitle:聚苯乙烯微塑料对肺炎克雷伯菌生长、生物膜形成和毒力的影响
- Author:
Zhenfu CHENG
1
,
2
;
Miao BAI
;
Yuchao BAI
;
Qianxiu ZHAO
;
Zhenghao WANG
;
Can ZHANG
;
Chuanfu ZHANG
Author Information
1. 安徽医科大学公共卫生学院卫生检验与检疫学系,合肥 230032
2. 中国人民解放军疾病预防控制中心,北京 100071
- Keywords:
microplastics;
Klebsiella pneumoniae;
oxidative stress;
biofilm formation;
virulence
- From:
Acta Universitatis Medicinalis Anhui
2024;59(10):1835-1841
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of polystyrene microplastics(PS-MPs)on the growth,activity,oxida-tive stress levels,biofilm formation and virulence of Klebsiella pneumoniae.Methods Klebsiella pneumoniae was exposed to PS-MPs at different concentrations(10,50 and 100 μg/ml)and particle sizes(0.1,1.0 and 5.0 μm),and the growth curves were measured.The bacterial activity was determined by CCK-8(cell counting kit-8).The level of intracellular reactive oxygen species(ROS)was determined by fluorescence probe.The biofilm forming ability was determined by crystal violet staining.Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the relative expression levels of biofilm-forming genes(luxS,mrkA,wbbM,pgaA,wzm)and viru-lence genes(ureA,uge,wabG,fimH).Results A high concentration(100 μg/ml)of 0.1 μm PS-MPs had a stronger inhibitory effect on the growth and activity of Klebsiella pneumoniae,and the intracellular ROS level signifi-cantly increased,indicating that smaller particle size and higher concentration of PS-MPs were more toxic to bacte-ria.PS-MPs of 100 μg/ml particle size groups(0.1,1.0 and 5.0 μm)significantly promoted the biofilm forma-tion of Klebsiella pneumoniae.The relative expression levels of biofilm formation related genes(luxS,mrkA,wbbM,pgaA,wzm)and virulence genes(ureA,uge,wabG,fimH)increased.Conclusion By inducing Kleb-siella pneumoniae to produce a high level of ROS,PS-MPs can cause oxidative stress,inhibit the growth and activi-ty of bacteria,and enhance the biofilm formation ability and virulence,thus affecting the biological characteristics of Klebsiae pneumoniae.
