Self Precipitation Analysis of Erhuang Powder and Its Effect on HeLa Cells
10.13748/j.cnki.issn1007-7693.20231828
- VernacularTitle:儿黄散自沉淀分析及其对HeLa细胞的作用研究
- Author:
Xinyi WANG
1
;
Shuyu ZHAO
1
;
Xiufeng YU
1
;
Feng GUAN
1
;
Weinan LI
1
;
Fengjuan HAN
2
;
Yanhong WANG
1
Author Information
1. School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China
- Publication Type:Journal Article
- Keywords:
Erhuang powder；self precipitation；Coptidis Rhizoma；Catechu；HeLa cells
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(12):1604-1614
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To explore the self precipitation source of Erhuang powder, determine the content of related components and its efficacy on HeLa cells.
METHODS
Bifurcation study to identify the main compatibility of precipitating. The self precipitation, supernatant and extract of Erhuang powder were analyzed by UHPLC-Q-Exactive MS. The main compounds in Coptidis Rhizoma and Catechu, catechin, epicatechin, epicberberine, coptisine, berberine and palmatine were selected as controls. A analysis method of UHPLC for self precipitation, supernatant and extract of Erhuang powder was established and the related components were quantitatively determined. The effects of self precipitation, supernatant and extract on HeLa cells were evaluated by MTT method and flow cytometry.
RESULTS
A slight flocculation precipitate appeared when the decoction of Erhuang powder was mixed in pairs, while a large amount of flocculation appeared when the decoction of Coptidis Rhizoma and Catechu water was mixed. The self precipitation, supernatant and extract samples contained 39 compounds, which were mainly alkaloids and phenolic acids. The contents of catechin and berberine in the 6 index components were mostly, which accounted for 73.56% of the total content of the index components in self precipitation and 61.89% of the total content of the index components in extract. Inhibition effect on HeLa cells: extract ≈ self precipitation > supernatant, and inducing apoptosis: self precipitate ≈ extract, supernatant had no apoptosis-inducing effect.
CONCLUSION
Coptidis Rhizoma-Catechu is the main compatible formula for precipitation formation. The self precipitation and extracts of Erhuang powder are mainly alkaloids and phenolic acids, among which berberine and catechin are high in content and can be used as representative components. The effect of self-precipitation and extract on HeLa cells was better than that of supernatant. This basically indicates that the self precipitation components and pharmacological effects of Erhuang powder are similar to those of the extract.
