Mechanism of Sanhuang Ointment in Treatment of Haemorrhoids Based on Network Pharmacology and Experimental Verification
10.13748/j.cnki.issn1007-7693.20230076
- VernacularTitle:基于网络药理学和实验验证的三黄膏治疗痔疮的作用机制研究
- Author:
Wenjie LI
1
;
Qian YU
1
;
Yi ZHENG
1
;
Yiliu YANG
1
;
Xinyue ZHANG
1
;
Guangzhe LI
2
;
Mingming YAN
2
Author Information
1. Changchun University of Chinese Medicine, Changchun 130117, China
2. Changchun University of Chinese Medicine, Changchun 130117, China；Jinlin Provincial Science and Technology Innovation Center of Health Food of Chinese Medicine, Changchun 130117, China
- Publication Type:Journal Article
- Keywords:
Sanhuang ointment；haemorrhoids；network pharmacology；molecular docking；experimental verification
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(12):1591-1603
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To predict the core ingredients, targets and signaling pathways of Sanhuang ointment in the treatment of haemorrhoids based on network pharmacology, and preliminarily discuss the mechanism of action preliminarily by means of molecular docking and experimental verification.
METHODS
Active ingredients, targets and haemorrhoid related targets of Sanhuang ointment were obtained through literature research and database screening. Association networks were constructed and core targets were screened by Cytoscape, and gene function analysis and pathway enrichment analysis were conducted. Autodock Vina was used for molecular docking verification, and the regulatory effects of Sanhuang ointment on core targets were verified by ELISA and immunohistochemistry.
RESULTS
The 77 active ingredients and 61 potential targets of Sanhuang ointment for treating haemorrhoids were collected. KEGG showed that Sanhuang ointment could regulate calcium signaling pathways, neuroactive ligand receptor interactions, cGMP-PKG pathway, etc. Affect 6 core targets of CGRP, NK-1R, TRPV1, VEGF, IL-6 and TNF-α. Molecular docking showed that Sanhuang ointment had a good affinity with target protein, and animal experiments verified that Sanhuang ointment could inhibit the expression of IL-6, CGRP, substance P, and promote the expression of VEGF.
CONCLUSION
Sanhuang ointment may act on CGRP, NK-1R and other targets, regulate Calcium signaling pathway and PI3K-Akt pathways, and exert analgesic and anti-inflammatory effects, promoting blood circulation and removing blood stasis to treat haemorrhoids.
