Expression and significance of miR-122-5p,miR-143-3p and inflammatory factors IL-6 and IL-10 in mice with nonalcoholic fatty liver disease induced by bisphenol A and high-fat diet
10.19405/j.cnki.issn1000-1492.2024.10.011
- VernacularTitle:miR-122-5p、miR-143-3p及炎症因子IL-6、IL-10在双酚A和高脂饮食诱导的小鼠非酒精性脂肪性肝病中的表达及意义
- Author:
Haiyan WANG
1
,
2
;
Yuxin ZHANG
;
Li ZHU
;
Ruiting MA
;
Yonghong ZHANG
Author Information
1. 内蒙古科技大学包头医学院研究生学院,包头 014040
2. 内蒙古科技大学包头医学院第一附属医院内分泌科,包头 014010
- Keywords:
nonalcoholic fatty liver disease;
bisphenol A;
miR-122-5p;
miR-143-3p;
inflammatory factors
- From:
Acta Universitatis Medicinalis Anhui
2024;59(10):1769-1776,1784
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of bisphenol A(BPA)exposure on liver lipid metabolism in C57BL/6J mice and uncover the mechanisms at work.Methods Male C57BL/6J mice,aged eight weeks,were stratified into six cohorts:a control group on a standard diet(ND),a group on a standard diet with low-dose BPA(BPA-50 ND),a group on a standard diet with high-dose BPA(BPA-500 ND),a control groupon a high-fat diet(HFD),a group on a high-fat diet with low-dose BPA(BPA-50 HFD),and a group on a high-fat diet with high-dose BPA(BPA-500 HFD).Dosages for the low-and high-dose BPA groups were 50 and 500 μg/(kg·d),respectively,administered via gavage over a duration of 12 weeks.Hepatic tissue underwent histological examination through he-matoxylin and eosin(HE)staining.Furthermore,the expression levels of miR-122-5p and miR-143-3p in hepatic tissue,in addition to interleukin(IL)-6 and IL-10 in peripheral serum,were quantitatively measured employing quantitative reverse transcription-polymerase chain reaction(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA),respectively.Results Histopathological analysis via HE staining indicated intact hepatic lobule archi-tecture in the ND group,whereas other groups displayed variable degrees of lipid droplet accumulation and damage to hepatic lobules.Notably,supplementation with BPA,particularly in conjunction with a high-fat diet,led to a progressive increase in IL-6 levels and a decrease in IL-10 levels in peripheral blood.In the context of a standard diet,an augmentation in BPA concentration corresponded with a decline in the expression of miR-122-5p and miR-143-3p.Conversely,within the high-fat diet cohort,enhanced BPA concentrations were associated with increased expressions of these microRNAs.Pearson correlation analysis disclosed a significant positive correlation between the expression of miR-122-5p and miR-143-3p and the level of IL-10 in the standard diet group(P＜0.01).In the high fat diet group,the expression level of miR-122-5p was positively correlated with the concentration of IL-6(P＜0.05),and the expression level of miR-143-3p was negatively correlated with the concentration of IL-10(P＜0.05).Conclusion BPA can induce the occurrence and progression of nonalcoholic fatty liver disease(NAFLD)by regulating the expression of miR-122-5p and miR-143-3p and regulating the levels of inflammatory factors IL-6 and IL-10.
- Full text:2024122511522840973miR-122-5p、mi...性脂肪性肝病中的表达及意义_王海燕.pdf
