The effect of miR-142-5p on oral squamous carcinoma and in angiogenesis
10.19405/j.cnki.issn1000-1492.2024.10.004
- VernacularTitle:miR-142-5p对口腔鳞状细胞癌及血管生成影响的实验研究
- Author:
Yixin LIU
1
;
Xiangyu LI
;
Mengci SHAO
;
Jing WANG
;
Wenhua XU
;
Yuanyin WANG
Author Information
1. 安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,合肥 230032
- Keywords:
non-coding RNA;
oral squamous cell carcinoma;
miR-142-5p;
angiogenesis;
proliferation;
migration
- From:
Acta Universitatis Medicinalis Anhui
2024;59(10):1713-1719,1728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR-142-5p in oral squamous cell carcinoma(OSCC)tis-sues and cell lines and its effects on oral squamous cell proliferation,migration,invasion and angiogenesis.Meth-ods Sixteen groups of oral tumour tissues and paraneoplastic tissues were collected,and qRT-PCR was applied to detect the expression of miR-142-5p in the tissues.The effects of miR-142-5p on cell proliferation,migration,and invasion were observed by cell counting kit-8(CCK-8),cloning,wound healing,Transwell,invasion assays,and the effect of miR-142-5p on angiogenesis was also detected by lumen formation assay.The expression of angiogene-sisrelated proteins vascular endothelial growth factor(VEGFA),vascular endothelial calreticulin(VE-cadherin),epithelial calreticulin(E-cadherin),matrix metalloproteinase 2(MMP2),and matrix metalloproteinase 9(MMP9)was detected by Western blot after overexpression of miR-142-5p.Results miR-142-5p was lowly ex-pressed in oral tumour tissues and cell lines.CCK-8 and clonogenic assays showed that miR-142-5p was inversely correlated with the proliferation of OSCC cells,wound healing and Transwell assays showed that miR-142-5p was inversely correlated with the migration of OSCC cells,and cell invasion assays showed that miR-142-5p was con-versely correlated with the invasion of OSCC cells.Analysis of lumen formation assay showed that overexpression of miR-142-5p reduced the tube length and nodes of HUVECs.Western blot assay showed that up-regulation of miR-142-5p inhibited the VEGFA,VE-cadherin,MMP2,MMP9 expression and promoted E-cadherin expression.Con-clusion Overexpression of miR-142-5p inhibites the proliferative,migratory and invasive effects of oral squamous carcinoma cells as well as angiogenesis,suggesting that miR-142-5p is a novel target for anti-tumour angiogenesis and against oral squamous carcinoma.
- Full text:202412251058100664miR-142-5p对口腔...癌及血管生成影响的实验研究_刘溢馨.pdf
