Porphyromonas gingivalis promotes autophagy in esophageal squamous cell carcinoma via the miR-21-5p/RASA1/ERK axis
10.19405/j.cnki.issn1000-1492.2024.09.017
- VernacularTitle:牙龈卟啉单胞菌通过miR-21-5p/RASA1/ERK信号通路促进食管鳞癌自噬
- Author:
Yubo DU
1
;
Yelin JIAO
;
Yueyue CHENG
;
Haodong CHENG
;
Yi RU
;
Gaofeng LIANG
;
Shegan GAO
;
Yijun QI
Author Information
1. 河南科技大学基础医学与法医学院,洛阳 471023
- Keywords:
esophageal squamous cell carcinoma;
Porphyromonas gingivalis;
autophagy;
miR-21-5p;
RASA1;
ERK
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1621-1628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular mechanism underlying Porphyromonas gingivalis(Pg)-induced autophagy in esophageal squamous cell carcinoma(ESCC).Methods After Pg infected KYSE70 cells and KYSE140 cells pretreated with siAtg7 or Chloroquin(CQ),Western blot was used to measure protein levels of Atg7,LC3-Ⅱ/LC3-Ⅰ,and p62;Immunofluorescent confocal imaging analysis was used to detect autophagosome and autolysosome;CCK-8 assay was used to test cell viability;Transwell assay was used for ESCC cell migration and invasion potentials.Likewise,miR-21-5p inhibitor,RASA1 overexpression plasmid,or U0126 were used to block miR-21-5p/RASA1/ERK signaling pathway prior to Pg infection,followed by the aforementioned methods.In addition,immunohistochemistry was used to examine Pg abundance and LC3 protein levels,and RT-PCR was used to evaluate miR-21-5p expression in ESCC and adjacent tissue samples,followed by correlation analyses be-tween Pg and LC3,and Pg and miR-21-5p.Results Pg infected KYSE70 cells and KYSE140 cells showed upreg-ulation Atg7 protein and LC3-Ⅱ/LC3-Ⅰ protein but downregulation of RASA1 protein and p62 protein,enhanced cell proliferation,migration,and invasion as well as immunofluorescent spots of red,green,and yellow in mRFP-GFP-LC3-labeled ESCC cells.Pretreatment with CQ or siAtg7 abolished the above alterations induced by Pg.Con-sistently,pretreatment with miR-21-5p inhibitor,U0126,or RASA1 overexpression plasmid also blocked Pg-stimu-lated autophagy.In ESCC samples,Pg abundance was correlated with upregulation of miR-21-5p and LC3.Con-clusion Pg promotes autophagy in esophageal squamous cell carcinoma via miR-21-5p/RASA1/ERK signaling pathway.
