Chitosan nanoparticles loaded withastilbin affect high glucose-induced ferroptosis of renal tubular epithelial cells by regulating MAPK14/HSP27
10.19405/j.cnki.issn1000-1492.2024.09.016
- VernacularTitle:壳聚糖纳米颗粒负载落新妇苷通过调控MAPK14/HSP27影响高糖诱导的肾小管上皮细胞铁死亡
- Author:
Yijun CHEN
1
;
Xiang LI
;
Jianzhuo HU
Author Information
1. 湖南中医药大学第二附属医院肾病内分泌科,长沙 410005
- Keywords:
astilbin;
chitosan nanoparticles;
mitogen-activated protein kinase 14;
heat shock protein 27;
ferrop-tosis;
diabetic nephropathy
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1610-1620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of astilbin(astilbin-CS-NPs)-loaded chitosan nanoparticles(CS-NPs)on high glucose(HG)-induced ferroptosis of renal tubular epithelial cells via regulating mitogen-activated protein kinase 14(MAPK14)/heat shock protein 27(HSP27).Methods HK-2 cells were divided into the following groups:normal glucose(NG)group,HG group,HG+astilbin-CS-NPs(0,5,10,20 mg/L)group,and under HG condition,pcDNA3.1-MAPK14 group,pcDNA3.1-MAPK14+astilbin-CS-NP group,si-HSP27 group,pcD-NA3.1-MAPK14+si-HSP27 group,and pcDNA3.1-MAPK14+si-HSP27+astilbin-CS-NPs group.Cell viability was detected using CCK-8 assay,cell apoptosis was detected via Tunel assay.Meanwhile,iron ion levels,lactate dehydrogenase(LDH)activity,glutathione(GSH)levels,and reactive oxygen species(ROS)levels were meas-ured using assay kits.Rat model of diabetic kidney disease(DKD)was constructed and intervened with astilbin-CS-NPs to explore the effects of astilbin-CS-NPs on DKD in vivo.Results Compared with the NG group,the HK-2 cell viability in the HG group was significantly reduced,apoptosis increased,iron ion levels,LDH activity,and ROS levels were significantly elevated,while GSH levels significantly decreased(all P＜0.05).Treatment with astilbin-CS-NPs significantly reversed the effects of HG on the biological behavior of HK-2 cells and ferroptosis-re-lated indicators.Additionally,compared to the pcDNA3.1 group,the pcDNA3.1-MAPK14 group showed increased ferroptosis,which was improved by knocking down HSP27 or co-intervention with astilbin-CS-NPs.In vivo experi-mental results showed that astilbin-CS-NPs could improve DKD rat kidney injury,inhibit iron ion levels and the ex-pression of MAPK14/HSP27.Conclusion Astilbin-CS-NPs may improve HG-induced ferroptosis of renal tubular epithelial cells via inhibition of MAPK14 and HSP27 expression.
- Full text:2024122421002852834壳聚糖纳米颗粒负载落新妇苷...诱导的肾小管上皮细胞铁死亡_陈毅君.pdf
