Dendrobine interferes with diabetic kidney disease by up-regulating HSP90AA1 to regulate immune cells
10.19405/j.cnki.issn1000-1492.2024.09.015
- VernacularTitle:石斛碱通过上调HSP90AA1调节免疫细胞干预糖尿病肾病
- Author:
Yunhui JIANG
1
;
Chuantie TANG
;
Jun LI
;
Nannan ZHANG
Author Information
1. 贵州中医药大学基础医学院,贵阳 550025
- Keywords:
dendrobine;
diabetic kidney disease;
HSP90AA1;
immune cells;
network pharmacology;
bioinformat-ics;
target
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1599-1609
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of dendrobine in the treatment of diabetic kidney disease(DKD)by intervening immunity using network pharmacology,bioinformatics and Western blot.Methods The targets of dendrobine were screened by Pharmmaper,Superpred and other databases.The disease targets of DKD were screened from GeneCards,OMIM and other databases.The immune-related targets were searched in Immport,In-nateDB and other databases.The intersection of the above three was obtained via Venn diagram.The PPI map of intersection targets was constructed by STRING software combined with Cytoscape software,and the core targets in the interaction network were mined by CytoHubba plug-in.The data set GSE142025 was obtained from GEO,and immune infiltration and WGCNA analysis were performed using the R language CIBERSORT package.GSE1009,GSE30122 and GSE142025 were used for gene differential expression analysis and ROC verification.The correla-tion between core targets and immune cell phenotype was analyzed.Molecular docking and molecular dynamics sim-ulation analysis were used to test the binding force of dendrobine to key targets.Western blot was used to detect the expression of HSP90AA1 in HK-2 cells.Results A total of 128 intersecting genes,including HSP90AA1,LCK and EGFR,were obtained,and the core targets involved HSP90AA1,LCK and STAT3;molecular docking and molecular dynamics simulation analyses showed that the protein of HSP90AA1 could bind well with dendrobine.Western blot experiments showed that compared with the control group,HSP90AA1 was significantly down-regula-ted in the high glucose group.Compared with the high glucose group,HSP90AA1 was significantly up-regulated in the dendrobine group.Conclusion Dendrobine can intervene in diabetic kidney disease by regulating the expres-sion of HSP90AA1.
