Characterization of Staphylococcus aureus strains with novel incomplete hemolytic phenotype
10.19405/j.cnki.issn1000-1492.2024.09.007
- VernacularTitle:新型不完全溶血表型金黄色葡萄球菌的微生物学特征
- Author:
Wei TANG
1
,
2
;
Guiyun LENG
;
Ju GAO
;
Yawu WANG
;
Jie YAO
;
Qiang ZHOU
;
Yuanhong XU
Author Information
1. 安徽医科大学第二附属医院检验科,合肥 230601
2. 安徽医科大学第一附属医院检验科,合肥 230022
- Keywords:
Staphylococcus aureus;
hemolysin;
hemolytic phenotype;
microbiological characteristics;
antibiotic resistance;
pathogenicity
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1535-1542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the microbiological characteristics of Staphylococcus aureus(S.aureus)with no-vel incomplete hemolytic phenotype(SIHP).Methods Hemolytic phenotypes were detected and categorized by u-sing the three-point inoculation method.A total of 11 novel SIHP and 33 randomly matched S.aureus with com-plete hemolytic phenotype(SCHP)were included.Antibiotic susceptibility test was performed using broth microdi-lution method.Coagulase test was performed with freeze-dried rabbit plasma.Catalase activity was detected by slide catalase test.Expression of hemolysin genes was detected by qRT-PCR.Toxicity to human red blood cells was as-sessed by microplate method.Microplate biofilm formation was measured using crystal violet staining method.Growth kinetic determination was performed through microcultivation assay.Results Compared with SCHP,the expression profiles of the four hemolysin genes(hla,hlb,hlc,and hld)in the new SIHP were different.The new SIHP had higher resistance rates to penicillin,oxacillin,gentamicin,quinolones,clindamycin,and trimethoprim-sulfamethoxazole.Furthermore,the new SIHP had stronger hemolytic toxicity,plasma coagulase activity,and bio-film formation ability.Additionally,the new SIHP grown faster in the logarithmic phase.Conclusion Taken to-gether,the microbiological characteristics of the new SIHP are different from those of SCHP,including stronger an-tibiotic resistance and pathogenicity,which should be paid more attention by clinicians.
- Full text:2024122419183345126新型不完全溶血表型金黄色葡萄球菌的微生物学特征_唐伟.pdf
