Comparison of imaging flow cytometry and immunofluorescence techniques for detection of Ahr entry in fibroblast-like synoviocytes
10.19405/j.cnki.issn1000-1492.2024.09.005
- VernacularTitle:成像流式细胞术和免疫荧光技术检测成纤维样滑膜细胞Ahr入核的比较
- Author:
Meng CHENG
1
;
Yi WANG
;
Weibo DONG
;
Wei WEI
;
Yan CHANG
Author Information
1. 安徽医科大学临床药理研究所、抗炎免疫药物教育部重点实验室,合肥 230032
- Keywords:
immunofluorescence;
imaging flow cytometry;
rheumatoid arthritis;
fibroblast-like synoviocytes;
aro-matic hydrocarbon receptor;
nucleation;
relevance
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1524-1528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the difference between imaging flow cytometry and immunofluorescence tech-niques for detecting Ahr entry in fibroblast-like synoviocytes.Methods Human-derived fibroblast-like synoviocytes line MH7A was cultured with 15％ DMEM.The nucleation levels of Ahr in MH7A were detected by laser scanning confocal microscopy and quantitative imaging flow cytometry(ImageStreamX Mark Ⅱ)in the blank control group,the Kyn group,and the Kyn+CH223191 group,respectively.The results of the two assay techniques were com-pared using nonparametric tests-correlation of the results.Results Compared with the blank control group,the Kyn and Kyn+CH223191 groups of MH7A cells increased Ahr nucleation ability as measured by immunofluores-cence technique and imaging flow cytometry(P＜0.05),and the three groups of results calculated by the two ex-perimental methods were consistent,with the R2 values of 0.863 8,0.928 7,and 0.901 8,respectively,which were statistically significant differences(P＜0.05).Conclusion Compared with the operation and results of im-munofluorescence experiments,imaging flow cytometry data processing is more complicated.However,the results are notably precise,avoiding the subjectivity of the experimenter and minimizing experimental inaccuracies.
