Mechanism of GP73 neutralizing antibody inhibiting MCD diet-induced nonalcoholic fatty liver disease
10.19405/j.cnki.issn1000-1492.2022.09.011
- Author:
Jialong Liu
1
,
2
;
Xiaoli Yang
1
,
2
;
Xuemiao Zhang
3
;
Xiaopan Yang
3
;
Congwen Wei
3
Author Information
1. Clinical Laboratory of The Third Medical Center of Chinese PLA General Hospital ,Anhui Medical University, Beijing 100039
2. Beijing Institute of Biotechnology,Academy ofMilitary Medical Sciences ,Beijing 100071
3. Beijing Institute of Biotechnology,Academy ofMilitary Medical Sciences ,Beijing 100071
- Publication Type:Journal Article
- Keywords:
golgi protein 73;
GP73 neutralizing antibody;
non⁃alcoholic fatty liver disease;
methionine⁃choline deficient
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1398-1403
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the function and mechanism of golgi protein 73(GP73) neutralizing antibody in Methionine-choline deficient(MCD) diet-induced non-alcoholic fatty liver disease(NAFLD).
Methods : Total cholesterol(TC),aspartate aminotransferase(AST),triglyceride(TG) and serum alanine aminotransferase(ALT) levels were detected after MCD diet and GP73 neutralizing antibody intervention. Lipid deposition in liver tissue was observed by oil red O staining; qRT-PCR was used to detect the gene expression ofACC1,HMGR,TIMP1andTGF-βin liver tissue; Western blotting was used to detect α-SMA and SREBP1 protein expression levels in liver tissues.
Results :GP73 neutralizing antibody could reduce the accumulation of serum ALT and AST(P<0.001) induced by MCD diet, and increase the levels of serum TG and TC(P<0.001);The gene expression levels ofHGMR(P<0.001),TGF-β(P<0.05),ACC1(P<0.01) and the protein expression levels of α-SMA(P<0.05) and SREBP1(P<0.05) in liver tissue significantly decreased, and the lipid deposition was also improved.
Conclusion : GP73 neutralizing antibody inhibits the formation of MCD-induced NAFLD by slowing down the progression of liver fibrosis and reducing the deposition of liver lipids.
- Full text:202412241555197963GP73中和抗体抑制MCD...导的非酒精性脂肪肝机制研究_刘家茏.pdf
