Co-loaded with miR-145/CPT nanoparticles for targeted delivery and magnetic resonance imaging in hepatocellular carcinoma
10.19405/j.cnki.issn1000-1492.2024.09.002
- VernacularTitle:脂质纳米颗粒共载miR-145/CPT肝癌靶向共递送及磁共振成像的初步研究
- Author:
Jing RONG
1
;
Tongtong LIU
;
Xiujuan YIN
;
Lei ZHANG
;
Xiao WANG
Author Information
1. 安徽医科大学第一附属医院放射科,合肥 230022
- Keywords:
magnetic resonance imaging;
lipid nanoparticles;
hepatocellular carcinoma;
molecular targeted thera-py;
lactobionic acid;
miR-145;
camptothecin;
combination therapy
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1501-1508
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop liver cancer-targeted nanoparticles(LA-CMGL)co-loaded with miR-145/camp-tothecin(CPT)and assess their targeting specificity,combined anti-tumor effects,and magnetic resonance imaging efficacy.Methods Laser scanning confocal microscopy and flow cytometry were utilized to evaluate the targeted uptake of lactobionic acid-modified and unmodified nanoparticles by HepG2 cells and HepaRG cells;quantitative polymerase chain reaction(qPCR)was employed to assess miR-145 content in tumor cells and tissues.The cytotox-icity of CPT,LA-CPT-LNPs and LA-CMGL on HepG2 cells were assessed using the CCK-8 assay.qPCR was also used to evaluate the effect of CPT,LA-CPT-LNPs and LA-CMGL on apoptosis of HepG2 cells.MRI was performed to measure the relaxation rate of LA-CMGL and evaluate its targeting imaging effect on liver cancer cells.Results The uptake rate of LA-CMGL by HepG2 cells surpassed that of CMGL significantly.The relative miR-145 content in liver cancer cells and mouse liver cancer tissues in the LA-CMGL group was markedly higher compared to free miR-145 and CMGL groups(P＜0.001).The apoptosis rate of HepG2 cells in the LA-CMGL group exceeded that in the CMGL group and CPT group(P＜0.01).At the same Gd3+concentration,the relaxation rate of LA-CMGL significantly surpassed that of Gd-DOTA,and the MRI signal of LA-CMGL in HepG2 cells markedly increased com-pared to CMGL and Gd-DOTA.Conclusion LA-CMGL exhibits promising liver cancer-targeted delivery,com-bined anti-tumor effects,and MRI liver cancer cell-targeted imaging,offering a novel avenue for combined drug/gene therapy for liver cancer.
- Full text:2024122415421437171脂质纳米颗粒共载miR-1...递送及磁共振成像的初步研究_容静.pdf
