Study on the expression of chemokine CXCL1 in infantile hemangioma tissues and the effect of exogenous CXCL1 on hemangioma stem cells
10.19405/j.cnki.issn1000-1492.2022.09.008
- Author:
Xiaoyue Zhai
1
;
Yao Wu
1
;
Yin Lou
1
;
Juan Xie
1
;
Honghong Li
1
;
Dongsheng Cao
1
Author Information
1. Dept of Plastic Surgery, The Second Afiliated Hospital of Anhui Medical University,Hefei 230601
- Publication Type:Journal Article
- Keywords:
chemokine;
CXCL1;
infantile hemangioma;
hemangioma stem cell;
proliferation
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1385-1388
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the expression of chemokine CXCL1 in proliferative infantile hemangioma (IH) , and to study the effect of exogenous CXCL1 on hemangioma stem cells (HemSCs) .
Methods :Immunohistochemistry was used to explore the expression of CXCL1 in proliferative IH specimens.Primary HemSCs were isolated from IH tissues by CD133 magnetic beads.5 groups of CXCL1 with different concentrations(0,10,20,50 and 100 ng/ml) were co-cultured with HemSCs, and the effects of exogenous CXCL1 on HemSCs were studied by cell viability and migration experiments.
Results : CXCL1 was expressed in the interstitial tissues of proliferative IH.The overall expression of CXCL1 in proliferative IH was low, but the expression of CXCL1 in the proliferative IH interstitial tissues was higher than that of the adjacent interstitial tissues.The CXCL1 positive area rate was(0.773±0.101)% in the tumor compared with(0.268±0.081)% in the adjacent tumor, and the difference was statistically significant(t=7.843,P<0.001).Exogenous CXCL1 promoted the proliferation of HemSCs, and there were statistical differences after adding different concentrations of CXCL1 to HemSCs for 24,48,and 72 h(F=14.610,P<0.001;F=14.430,P<0.001;F=5.388,P<0.01).But the exogenous CXCL1 did not affect the migration ability of HemSCs.
Conclusion:The expression of CXCL1 in proliferative IH interstitial tissues is higher than that in adjacent interstitial tissues, and exogenous CXCL1 promotes the proliferation of HemSCs.
- Full text:2024122415372864563趋化因子CXCL1在增殖期...CL1对血管瘤干细胞的影响_翟晓玥.pdf
