Up-regulation of tryptophan-2,3-dioxygenase 2 mediates the role of kynurenine metabolism in adjuvant arthritis in rats
10.19405/j.cnki.issn1000-1492.2022.09.006
- Author:
Chengyan Jia
1
;
Ping Han
1
;
Yueye Wang
1
;
Yuan Xu
1
;
Hepeng Xu
1
;
Ayuan Chen
1
;
Wei Wei
1
;
Yan Chang
1
Author Information
1. Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory ofAnti⁃inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center ofAnti⁃Inflammatory and Immune Medicine , Rheumatoid Arthritis Research Center,Anhui Medical University,Hefei 230032
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
adjuvant arthritis;
tryptophan⁃2 ,3 ⁃dioxygenase 2;
kynurenine;
tryptopha
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1374-1379
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the changes of tryptophan-2,3-dioxygenase 2(TDO2) expression and its mediated kynurenine(Kyn) pathway in different inflammatory stages(initial period, peak period and remission period) of adjuvant arthritis in rats.
Methods:A rat model of AA was established.AA rats were sacrificed at different stages of inflammation.The pathological changes of joint synovium and liver were observed by HE staining; TDO2 expression in joint synovial tissue and liver tissue was detected by immunohistochemistry; High performance liquid chromatography was used to detect tryptophan(Trp) and Kyn levels in serum and liver.
Results : Compared with the normal group, AA rats showed similar clinical and pathological features to RA.The expression of TDO2 in rat joint synovium and liver tissue increased during the peak period and remission period(P<0.05).Trp levels in serum of AA rats at each inflammation stage decreased, and Kyn levels increased in the initial stage(P<0.01);Trp levels in the liver tissues of rats at the peak period decreased, Kyn levels increased, and Kyn/Trp ratio increased(P<0.01).
Conclusion:TDO2 may play an important role in the peak period of AA,and mediate Kyn metabolism to participate in the occurrence and development of RA.
- Full text:2024122415240488543色氨酸-2,3-双加氧酶2...鼠佐剂性关节炎中的作用研究_贾成艳.pdf
