Artesunate affects the proliferation and migration of HCC cells by regulating the PI3K/AKT pathway through FABP5
10.19405/j.cnki.issn1000-1492.2022.09.005
- Author:
Qingsen Wang
1
,
2
;
Jing Wu
1
,
2
,
3
;
Jiawei Zhou
1
,
2
;
Yafeng Liu
1
,
2
;
Anqi Cheng
1
,
2
;
Dong Hu
1
,
2
,
3
Author Information
1. Dept of Medical College, Anhui University of Science and Technology,Huainan 232001
2. Anhui Occupational Health and Safety Engineering Laboratory, Anhui University of Science and Technology,Huainan 232001
3. Key Laboratory of Industrial Dust Control and Occupational Safety and Health ,Ministry of Education , Anhui University of Science and Technology,Huainan 232001
- Publication Type:Journal Article
- Keywords:
network pharmacology;
artesunate;
hepatocellular carcinoma;
molecular docking;
tumor cell proliferation and migration
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1367-1374
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the potential targets, pathways and related mechanisms of artesunate(ART) in the treatment of hepatocellular carcinoma(HCC) based on network pharmacology and Bioinformatics.
Methods:The potential targets of ART were screened through pharmmapper database, PPI network was constructed and visual analysis was performed.The data of 424 HCC samples were downloaded from TCGA database, the data information of potential target genes were screened, and the differentially expressed target genes were analyzed.The key target genes were screened by univariate-multivariate COX regression analysis.Molecular docking software was used to conduct molecular docking between ART and key target genes.Differential expression and survival analysis of key target genes were performed using network database GEPIA2. KEGG pathway enrichment analysis was performed by R language. The effects of ART on the proliferation and migration of HCC cells were verified by MTS assay and scratch test. The expression of FABP5 in HCC cells was verified by qPCR and Western blot. Western blot was used to verify the protein changes of PI3K/AKT pathway.
Results : A total of 282 potential target genes were screened by network pharmacology. Finally,three key target genes were screened out. The survival analysis of FABP5 in HCC was statistically significant(P<0. 01). Molecular docking showed that ART and FABP5 had the highest binding energy. Pathway enrichment analysis showed that FABP5 was mainly enriched in PI3K/AKT signaling pathway. Cell experiments verified that ART could inhibit the expression level of FABP5,that ART could regulate the PI3K/AKT pathway,and that ART could inhibit the proliferation and migration of HCC cells.
Conclusion :FABP5 may regulate the PI3K/AKT pathway by inhibiting FABP5 and thus affect HCC cell proliferation and migration,and FABP5may serve as a new target gene for ART therapy of HCC.
- Full text:2024122415140789554青蒿琥酯通过FABP5调节...路影响肝癌细胞的增殖和迁移_王清森.pdf
