Effect of Rhei Radix et Rhizoma Before and After Steaming with Wine on Intestinal Flora and Immune Environment in Constipation Model Mice
10.13422/j.cnki.syfjx.20241167
- VernacularTitle:大黄酒蒸前后对便秘模型小鼠肠道菌群及免疫环境的影响
- Author:
Yaya BAI
1
;
Rui TIAN
1
;
Yajun SHI
1
;
Chongbo ZHAO
1
;
Jing SUN
1
;
Li ZHANG
2
;
Yonggang YAN
1
;
Yuping TANG
1
;
Qiao ZHANG
1
Author Information
1. Shaanxi Engineering Technology Research Center of Chinese Herbal Pieces，Shaanxi Qinling Application Development and Engineering Research Center of Chinese Herbal Medicine，Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine（TCM） for TCM Compatibility， Shaanxi University of Chinese Medicine，Xi′an712046，China
2. Hanlin College，Nanjing University of Chinese Medicine，Taizhou 225300，China
- Publication Type:Journal Article
- Keywords:
Rhei Radix et Rhizoma;
steaming with wine;
constipation model mice;
intestinal flora;
cellular immunity;
inflammatory factors;
gastrointestinal hormones
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(2):192-199
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study on the different therapeutic effects and potential mechanisms of Rhei Radix et Rhizoma（RH） before and after steaming with wine on constipation model mice. MethodsFifty-four male ICR mice were randomly divided into control group， model group， lactulose group（1.5 mg·kg-1）， high， medium and low dose groups of RH and RH steaming with wine（PRH）（8， 4， 1 g·kg-1）. Except for the control group， the constipation model was replicated by gavage of loperamide hydrochloride（6 mg·kg-1） in the other groups. After 2 weeks of modeling， each administration group was gavaged with the corresponding dose of drug solution， and the control and model groups were given an equal volume of normal saline， 1 time/d for 2 consecutive weeks. After administration， the feces were collected for 16S rRNA sequencing， the levels of gastrin（GAS）， motilin（MTL）， interleukin-6（IL-6）， γ-interferon（IFN-γ） in the colonic tissue were detected by enzyme-linked immunosorbent assay（ELISA）， the histopathological changes of colon were observed by hematoxylin-eosin（HE） staining， flow cytometry was used to detect the proportion changes of CD4+， CD8+ and regulatory T cell（Treg） in peripheral blood. ResultsCompared with the control group， the model group showed significantly decrease in fecal number in 24 h， fecal quality and fecal water rate（P<0.01）， the colon was seen to have necrotic shedding of mucosal epithelium， localized intestinal glands in the lamina propria were degenerated， necrotic and atrophied， a few lymphocytes were seen to infiltrate in the necrotic area in a scattered manner， the contents of GAS and MTL， the proportions of CD4+， CD8+ and Treg were significantly reduced（P<0.01）， the contents of IL-6 and IFN-γ were significantly elevated（P<0.05， P<0.01）. Compared with the model group， the fecal number in 24 h， fecal quality and fecal water rate of high-dose groups of RH and PRH were significantly increased（P<0.05， P<0.01）， the pathological damage of the colon was alleviated to varying degrees， the contents of GAS， MTL， IL-6 and IFN-γ were significantly regressed（P<0.05， P<0.01）， and the proportions of CD4+ and CD8+ were significantly increased（P<0.01）， although the proportion of Treg showed an upward trend， there was no significant difference. In addition， the results of intestinal flora showed that the number of amplicon sequence variant（ASV） and Alpha diversity were decreased in the model group compared with the control group， and there was a significant difference in Beta diversity， with a decrease in the relative abundance of Lactobacillus and an increase in the relative abundances of Bacillus and Helicobacter. Compared with the model group， the ASV number and Alpha diversity were increased in the high-dose groups of RH and PRH， and there was a trend of regression of Beta diversity to the control group， the relative abundance of Lactobacillus increased， and the relative abundances of Bacillus and Helicobacter decreased. ConclusionRH and PRH can improve dysbacteriosis， promote immune system activation， inhibit the release of inflammatory factors for enhancing the gastrointestinal function， which may be one of the potential mechanisms of their therapeutic effect on constipation.
