Serum Metabolomics of Simulated Weightless Rats Treated with Taikong Yangxin Pills
10.13422/j.cnki.syfjx.20241219
- VernacularTitle:太空养心丸干预模拟失重大鼠的血清代谢组学分析
- Author:
Xiaodi LIU
1
;
Xuemei FAN
2
;
Yiming WANG
1
;
Mengjia YAN
1
;
Yongzhi LI
3
;
Jiaping WANG
3
;
Junlian LIU
3
;
Guoan LUO
1
Author Information
1. School of Traditional Chinese Medicine， Guangdong Pharmaceutical University， Guangzhou 510006， China
2. School of Pharmaceutical Sciences， Tsinghua University， Beijing 100084， China
3. China astronaut Research and Training Center，Beijing 100094， China
- Publication Type:Journal Article
- Keywords:
Taikong Yangxin pills;
model of simulated weightlessness;
metabolomics;
ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF/MS）;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(2):147-153
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of Taikong Yangxin Pills on the metabolism of simulated weightless rats based on metabolomics and discuss the metabolism mechanism. MethodsIn the simulated space capsule environment on the ground， the rat model of simulated weightlessness was established by the tail suspension method. Rats were randomly grouped as follows： out-of-capsule control， in-capsule control， model， and high （3.0 g·kg-1） and low （1.5 g·kg-1） doses of Taikong Yangxin Pills， and they were administrated with corresponding drugs by gavage for 28 days. The serum levels of endogenous metabolites in rats were determined by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF/MS）. The obtained data were processed by principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） to screen for differential metabolites and potential biomarkers. MetaboAnalyst 5.0 was used for pathway enrichment analysis to explain the metabolic regulation mechanism of the drug. ResultsCompared with the out-of-capsule control group， the in-capsule control group showed elevated levels of thirteen metabolites， including 14-hydroxyhexadecanoic acid， linoleic acid， and α-linolenic acid （P<0.05）， which suggested that the space capsule environment mainly affected the metabolism of α-linolenic acid and linoleic acid in the rats. Compared with the in-capsule control group， the model group showed lowered levels of fourteen metabolites， including 4-imidazolone-5-propionic acid， isocitric acid/citric acid， and L-tyrosine （P<0.05）， which were recovered after the treatment with Taikong Yangxin pills （P<0.05）. The pathway enrichment analysis revealed that weightlessness induced by tail suspension and drug intervention mainly involved the phenylalanine， tyrosine， and tryptophan biosynthesis， tyrosine metabolism， histidine metabolism， and citric acid cycle. ConclusionThe simulated space capsule environment and simulated weightlessness induced by tail suspension can both affect the metabolism level of rats. Taikong Yangxin pills can ameliorate the metabolic abnormality in the rat model of weightlessness by regulating various amino acids and energy metabolism-related pathways.
