Determination of resorcinol， ferulic acid， phenylethylresorcinol and benzoyl peroxide in freckle whitening cosmetics by high performance liquid chromatography
10.19428/j.cnki.sjpm.2024.24090
- VernacularTitle:高效液相色谱法测定祛斑美白类化妆品中的间苯二酚、阿魏酸、苯乙基间苯二酚和过氧化苯甲酰
- Author:
Ruoxuan YANG
1
;
Jing HAN
1
;
Rong ZHENG
1
;
Xingsheng PENG
1
;
Yong XU
1
;
Shaorong LUAN
2
Author Information
1. Shanghai Institute for Food and Drug Control, National Medical Products Administration Key Laboratory for Monitoring and Evaluation of Cosmetics, Shanghai201203, China
2. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai200237, China
- Publication Type:Journal Article
- Keywords:
resorcinol;
ferulic acid;
phenethylresorcinol;
benzoyl peroxide;
high performance liquid chromatography;
cosmetics
- From:
Shanghai Journal of Preventive Medicine
2024;36(10):997-1002
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish an analytical method for the determination of resorcinol, ferulic acid, phenethylresorcinol and benzoyl peroxide in freckle whitening cosmetics by high performance liquid chromatography (HPLC), to provide data support for the establishment of cosmetics inspection methods and technical support for the supervision of the cosmetics industry. MethodsThe analytes in samples were extracted by ultrasonic acetonitrile after methanol vortex, and then filtered by centrifugation and microporous filter membrane. Finally, the analytes were separated with a SVEA C4 column (250 mm×4.6 mm, 5 μm). The mobile phase was composed of 0.1% phosphoric acid solution -acetonitrile, and the gradient elution was applied, with a flow rate of 1.0 mL·min-1. The samples were detected by an ultra-violet detector and quantified by external standard method. ResultsResorcinol, ferulic acid, phenylethylresorcinol and benzoyl peroxide showed good linearity in the experimental range with r>0.999. HPLC was used to investigate the positive spiked recoveries of ferulic acid or phenylethylresorcinol with different matrices. The results showed that the recoveries were all in the range of 87.48% to 101.00%, and the relative standard deviations were all in the range of 3.4% to 4.1%. Furthermore, HPLC also examined the blank matrix spiked with the recoveries ranged from 93.26% to 107.66%, with the relative standard deviation of 0.90% to 2.90%. The limits of detection ranged from 0.000 8% to 0.002%. Among the30 batches of standard freckle whitening cosmetics determined, 6 batches of which were detected with phenethylresorcinol and 1 batch with ferulic acid. ConclusionHPLC is a method with rapidity, simplicity, and high sensitivity, which is suitable for the simultaneous determination of resorcinol, ferulic acid, phenethylresorcinol and benzoyl peroxide in commercially available cosmetics.
