Protective Effect of Xuebijing on Lung Injury in Rats with Severe Acute Pancreatitis by Blocking FPRs/NLRP3 Inflammatory Pathway
10.13422/j.cnki.syfjx.20241414
- VernacularTitle:血必净阻断FPRs/NLRP3炎症途径对重症急性胰腺炎大鼠肺损伤的保护作用
- Author:
Guixian ZHANG
1
;
Dawei LIU
1
;
Xia LI
1
;
Xijing LI
1
;
Pengcheng SHI
1
;
Zhiqiao FENG
2
;
Jun CAI
1
;
Wenhui ZONG
1
;
Xiumei ZHAO
1
;
Hongbin LIU
3
Author Information
1. Tianjin Institute of Medical and Pharmaceutical Science， Tianjin 300020， China
2. Tianjin Chase Sun Pharmaceutical Co. Ltd.， Tianjin 301700， China
3. Health Commission of Heping District， Tianjin 300040， China
- Publication Type:Journal Article
- Keywords:
severe acute pancreatitis;
Xuebijing injection;
formyl peptide receptor 1;
formyl peptide receptor 2;
nucleotide-binding oligomerization domain-like receptor 3
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(1):113-120
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the therapeutic effect of Xuebijing injection （XBJ） on severe acute pancreatitis induced acute lung injury （SAP-ALI） by regulating formyl peptide receptors （FPRs）/nucleotide-binding oligomerization domain-like receptor 3 （NLRP3） inflammatory pathway. MethodsSixty rats were randomly divided into a sham group， a SAP-ALI model group， low-， medium-， and high-dose XBJ groups （4， 8， and 12 mL·kg-1）， and a positive drug （BOC2， 0.2 mg·kg-1） group. For the sham group， the pancreas of rats was only gently flipped after laparotomy， and then the abdomen was closed， while for the remaining five groups， SAP-ALI rat models were established by retrograde injection of 5% sodium taurocholate （Na-Tc） via the biliopancreatic duct. XBJ and BOC2 were administered via intraperitoneal injection once daily for 3 d prior to modeling and 0.5 h after modeling. Blood was collected from the abdominal aorta 6 h after the completion of modeling， and the expression of interleukin （IL）-1β， IL-6， and tumor necrosis factor-α （TNF-α） in plasma was measured by enzyme-linked immunosorbent assay （ELISA）. The amount of ascites was measured， and the dry-wet weight ratios of pancreatic and lung tissue were determined. Pancreatic and lung tissue was taken for hematoxylin-eosin （HE） staining to observe pathological changes and then scored. The protein expression levels of FPR1， FPR2， and NLRP3 in lung tissue were detected by the immunohistochemical method. Western blot was used to detect the expression of FPR1， FPR2， and NLRP3 in lung tissue. Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was used to detect the mRNA expression of FPR1， FPR2， and NLRP3 in lung tissue. ResultsCompared with the sham group， the SAP-ALI model group showed significantly decreased dry-wet weight ratio of lung tissue （P<0.01）， serious pathological changes of lung tissue， a significantly increased pathological score （P<0.01）， and significantly increased protein and mRNA expression levels of FPR1， FPR2， and NLRP3 in lung tissue （P<0.01）. After BOC2 intervention， the above detection indicators were significantly reversed （P<0.01）. After treatment with XBJ， the groups of different XBJ doses achieved results consistent with BOC2 intervention. ConclusionXBJ can effectively improve the inflammatory response of the lungs in SAP-ALI rats and reduce damage. The mechanism may be related to inhibiting the expression of FPRs and NLRP3 in lung tissue， which thereby reduces IL-1β and simultaneously antagonize the release of inflammatory factors IL-6 and TNF-α.
