Effect of Shenge Bushen Capsules and Its Polysaccharides and Flavonoids on Precocious Puberty in Young Mice
10.13422/j.cnki.syfjx.20241603
- VernacularTitle:参葛补肾胶囊及其多糖黄酮对幼龄小鼠性早熟的影响
- Author:
Hong SUN
1
;
Fan LEI
2
;
Chenggong LI
3
;
Shixian HU
3
;
Weihua WANG
4
;
Bin REN
3
;
Juan HAO
3
;
Rui LUO
1
;
Lijun DU
2
Author Information
1. Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100094，China
2. School of Life Sciences， Tsinghua University，Beijing 100084，China
3. Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.，Urumqi 830000，China
4. Pharmacy Technology Center，Tsinghua University，Beijing 100084，China
- Publication Type:Journal Article
- Keywords:
Shenge Bushen capsules;
Pseudostellaria Radix polysaccharide;
total flavonoids;
precocious puberty;
young mice;
estradiol;
testosterone
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(1):95-103
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Shenge Bushen Capsules （SBC） on sexual development in normal 3-week-old mice. MethodsThe experiment consisted of two parts. In the first part， mice were divided into four groups： The control group and the low， medium， and high-dose SBC groups （234.7， 469.4， 938.7 mg·kg-1， respectively）. In the second part， mice were divided into four groups： Control group， Pseudostellariae Radix polysaccharide （PRP） group， total flavonoids group， and SBC group， all receiving a dose of 469.4 mg·kg-1. After 7 days of administration， the vaginal opening of female mice and the descent of testes and scrotum in male mice， as well as the ovarian and testicular organ indices， were observed. After 4 weeks of administration， female and male mice were housed together for 2 days， and the pregnancy rate of females was monitored. After delivery， the pregnant female mice continued receiving the treatment for 4 weeks， and the sexual development of their offspring， including vaginal opening， testicular descent， and organ indices of ovaries and testes， was observed. Serum sex hormones were measured by enzyme-linked immunosorbent assay （ELISA）， and the expression of gonadotropin-releasing hormone （GnRH） and growth hormone （GH） proteins in the hypothalamus was assessed by Western blot. ResultsCompared with the control group， there was no significant effect on the vaginal opening of female mice or the descent of testes in male mice after 7 days of SBC administration. After 4 weeks of administration， the pregnancy rate in the low-dose group was significantly reduced （P<0.05）， but no significant effects were observed in the other groups. The three doses of SBC did not significantly affect the ovarian or testicular organ indices， and there was no significant upregulation in the expression of GnRH or GH in the hypothalamus. The primary component of SBC， Pseudostellariae Radix polysaccharide， significantly reduced the vaginal opening in female mice after 7 days of administration （P<0.05）. After 4 weeks， the serum estradiol levels of non-pregnant female mice were decreased （P<0.05）， but there was no significant effect on the expression of GnRH or GH proteins in the hypothalamus of either male or female mice. Additionally， there were no significant effects on precocious puberty indicators， such as vaginal opening and testicular descent， in the offspring mice. ConclusionSBC does not significantly promote precocious puberty in young mice， and it does not have any noticeable effects on the pregnancy rate of adult mice or the sexual development of their offspring.
