Effect of Serum Containing Buyang Huanwutang on Podocytes Induced by High Glucose in Mice
10.13422/j.cnki.syfjx.20241036
- VernacularTitle:补阳还五汤含药血清对高糖诱导小鼠足细胞的影响
- Author:
Linlin ZHENG
1
;
Dengzhou GUO
1
Author Information
1. Graduate School， Hebei University of Chinese Medicine，Shijiazhuang 050091，China
- Publication Type:Journal Article
- Keywords:
Buyang Huanwutang;
diabetes kidney disease;
podocyte;
ferroptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(1):64-73
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of serum containing Buyang Huanwutang on podocyte injury induced by high glucose in mice， and to explore its potential mechanism. MethodsMouse podocytes （MPC5） were cultured in vitro， and the optimal intervention concentration and time of serum containing Buyang Huanwutang were screened. Cells were divided into normal group （5.5 mmol·L-1 glucose）， isotonic group （5.5 mmol·L-1 glucose+24.5 mmol·L-1 Mannitol）， high glucose group （30 mmol·L-1 glucose）， blank serum group （30 mmol·L-1 glucose+20% blank serum） and Buyang Huanwutang（30 mmol·L-1 glucose+10% serum containing）. FerroOrange fluorescent probe was used to detect the level of iron （Fe2+） in cells. The level of reactive oxygen species （ROS） in cells was detected by Hoechst staining. The levels of glutathione （GSH） and malondialdehyde （MDA） in cells were detected by the kit. Enzyme-linked immunosorbent assay （ELISA） was used to determine the level of 4-hydroxynonenal （4-HNE）. Western blot was used to detect Desmin， podocyte hole membrane protein （Nephrin）， Podocalyxin， long-chain acyl-CoA synthetase 4（ACSL4）， member 11 of solute carrier family 7 （SLC7A11）， glutathione peroxidase 4（GPX4）， nuclear transcription factor E2-related factor 2（Nrf2） and heme oxygenase -1（HO-1）. The mRNA expression levels of Desmin， Nephrin， Podocalyxin， ACSL4， SLC7A11， GPX4， Nrf2 and HO-1 were detected by real-time fluorescence quantitative polymerase chain reaction. ResultsCompared with the normal control group， the expressions of Desmin and ACSL4， the levels of Fe2+ and ROS， and the contents of MDA and 4-HNE in the high glucose group increased significantly， and the expressions of GSH， Nephrin， Podocalyxin， SLC7A11， GPX4， Nrf2 and HO-1 decreased（P<0.05， P<0.01）. There was no significant change in the above results in isotonic group. Compared with the high glucose group， the expressions of Desmin and ACSL4， the levels of Fe2+ and ROS， and the contents of MDA and 4-HNE in Buyang Huanwutang-containing serum group and Fer-1 group decreased significantly， and the expressions of GSH， Nephrin， Podocalyxin， SLC7A11， GPX4， Nrf2 and HO-1 increased（P<0.05， P<0.01）. There was no significant change in the above results in blank serum group. ConclusionBuyang Huanwutang medicated serum can alleviate podocyte injury induced by high glucose， and its mechanism is related to regulating Nrf2/HO-1 pathway and inhibiting ferroptosis.
