Tnfrsf11a Cre mediates YFP labeling some of tissue macrophages
10.19405/j.cnki.issn1000-1492.2022.09.001
- Author:
Fengjiao Yang
1
;
Ziwei Huang
1
;
Dianyuan Zhao
2
,
3
;
Long Xu
1
;
Li Tang
1
Author Information
1. Dept ofImmunology,School of Basic Medical Sciences ,Anhui Medical University,Hefei 230032
2. Institute of Lifeomics ,Academy ofMilitary Medical Sciences ,Academy ofMilitary Sciences ,Beijing 102206
3. State Key Laboratory of Proteomics ,Beijing Proteome Research Center,National Center for Protein Sciences ,Beijing 102206
- Publication Type:Journal Article
- Keywords:
Tnfrsf11aCre;
Rosa26yfp;
tissue macrophages;
brain microglia;
cre recombinase;
reporter mouse;
flow cytometry
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1345-1349
- CountryChina
- Language:Chinese
-
Abstract:
Objective : Tnfrsf11 a Cre Rosa26 yfp reporter gene mice were prepared to determine the efficiency of Cre-mediated recombination using flow cytometry in different tissue-resident macrophages.
Methods :TheTnfrsf11 a Cre Rosa26 yfp reporter mice were generated by crossingTnfrsf11 a Cre mice withRosa26 yfp mice and identified by PCR.Brain microglia, liver macrophages, kidney macrophages, alveolar macrophages and spleen macrophages were separated from adultTnfrsf11 a Cre Rosa26 yfp reporter mouse and yellow fluorescent protein(YFP) labeling efficiency was analyzed by flow cytometry.
Results : YFP expression percentage was about 91.27% of brain microglia inTnfrsf11 a Cre Rosa26 yfp reporter mice, but liver, spleen, and alveolar macrophages were inefficiently targeted(63.60%,69.66%,32.76%).
Conclusion:Tnfrsf11 a Cre mice were qualified as conditional knockout model mice of brain microglia andTnfrsf11 a Cre Rosa26 yfp reporter mice can be used as a tool for conditional knockout of brain microgliain vivo.
- Full text:2024122410532095422Tnfrsf11a~(Cr...素蛋白有效标记脑小胶质细胞_杨凤娇.pdf
