Effect of Myricetin on Immune Function in Rats with Inflammatory Bowel Disease by Regulating the cAMP/PKA/CREB Signaling Pathway
10.13748/j.cnki.issn1007-7693.20233703
- VernacularTitle:杨梅素调节cAMP/PKA/CREB信号通路对炎症性肠病大鼠免疫功能的影响
- Author:
Yani ZHOU
1
;
Ruonan LI
2
Author Information
1. College of Health Management, Shangluo University, Shangluo 726000, China；Shangluo Health Industry Engineering Technology Research Center, Shangluo 726000, China
2. College of Health Management, Shangluo University, Shangluo 726000, China
- Publication Type:Journal Article
- Keywords:
myricetin ；cyclic adenosine monophosphate/protein kinase A/cyclic adenosine monophosphate responsive component binding protein(AMP/PKA/CREB) signaling pathway；inflammatory bowel disease ； immune function
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(11):1456-1463
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To investigate the effect of myricetin(Myr) on immune function in rats with inflammatory bowel disease(IBD) by regulating the cAMP/PKA/CREB signaling pathway.
METHODS
IBD rat models were established and separated into control group, model group, low, medium, and high dose Myr(Myr-L, Myr-M, Myr-H, 28, 56, 112 mg·kg−1·d−1 Myr) groups, and high dose Myr+PKA inhibitor H89(Myr-H+H89 112 mg·kg−1·d−1 Myr+7 mg·kg−1·d−1 H89) group. The disease activity index(DAI) of rats was scored; immune function indicators and colon length were measured; the levels of IL-6, IL-17A, TNF-α, and cAMP in serum were determined by the kit; the pathological changes of colon tissue were observed by HE staining; the proportion of Treg cells was determined by flow cytometry; immunohistochemistry was used to detect the expression of MPO in colon tissue; Western blotting was used to determine cAMP/PKA/CREB signaling pathway related proteins.
RESULTS
Compared with the control group, the colon tissue cells in the model group were disorderly arranged, with a large number of inflammatory cell infiltration, severe ulceration, a large number of cell necrosis, mucosal edema, the DAI score, IL-6, TNF-α, and IL-17A levels, spleen coefficient, thymus coefficient, and MPO optical density values were obviously increased(P<0.05), the colon length, Treg cell ratio, cAMP concentration, p-PKA/PKA, and p-CREB/CREB levels were obviously reduced(P<0.05). Compared with the model group, the arrangement of colon tissue cells in the Myr-L, Myr-M, and Myr-H groups was relatively neat; mucosal edema inflammatory cell infiltration, cell necrosis and ulcer phenomenon were reduced; the DAI score, IL-6, TNF-α, and IL-17A levels, spleen coefficient, thymus coefficient, and MPO optical density values were gradually reduced(P<0.05); the colon length, Treg cell ratio, cAMP concentration, p-PKA/PKA, and p-CREB/CREB levels were gradually increased(P<0.05). Compared with the Myr-H group, the pathological changes in the colon tissue of the Myr-H+H89 group worsened, the DAI score, IL-6, TNF-α, and IL-17A levels, spleen coefficient, thymus coefficient, and MPO optical density values were obviously increased(P<0.05), the colon length, Treg cell ratio, cAMP concentration, p-PKA/PKA, and p-CREB/CREB levels were obviously reduced(P<0.05).
CONCLUSION
Myr may inhibit inflammation levels, regulate immune function, and exert protective effects on IBD rats by activating the cAMP/PKA/CREB signaling pathway.
