Rapid Analysis of Chemical Constituents in Rubus Sachalinensis Leveille of Mongolian Medical Herb by HPLC-Q-Exactive-MS/MS
10.13748/j.cnki.issn1007-7693.20233603
- VernacularTitle:HPLC-Q-Exactive-MS/MS快速分析蒙药材悬钩子木中的化学成分
- Author:
Jing LI
1
;
Na LI
2
;
Baoquan BAO
1
;
Dongning HAN
1
;
Ping ZHANG
1
Author Information
1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010110, China
2. Central Hospital of Xilin Gol League, Inner Mongolia, Xilin Gol League 026000, China
- Publication Type:Journal Article
- Keywords:
Rubus sachalinensis Leveille；HPLC-Q-Exactive-MS/MS；chemical constituents；compositional attribution
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(11):1447-1455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To rapidly analyze the chemical constituents of Rubus sachalinensis Leveille by HPLC-Q-Exactive-MS/MS.
METHODS
Chromatographic separation was carried out on CAPCELL PAK MGII C18(4.6 mm×250 mm, 5 μm) column at the temperature of 30 ℃. The mobile phase was acetonitrile-0.1% formic acid by gradient elution, with a flow rate of 1.0 mL∙min−1, and the injection volume of 20 µL. The MS spectrum was acquired in negative ion modes using HESI ion source.
RESULTS
The molecular and structural formulae of the compounds were determined based on the exact mass number and ChemSpider and PubChem databases. By comparing the retention time of the corresponding reference standards and those reported in the literature, primary mass spectra, and secondary mass spectrometry pyrolysis fragments, combined with fragmentation regularity of such compounds, a total of 71 compounds were identified from Rubus sachalinensis Leveille, including 30 organic acids, 22 flavonoids, 7 triterpenoid saponins, 5 coumarins, 1 lignan, 1 gallotannin and 2 aromatic compounds.
CONCLUSION
This method can quickly and accurately identify the complex chemical constituents in Rubus sachalinensis Leveille, and provide scientific basis for the basic research on the medicinal substances of Rubus sachalinensis Leveille.
