Effect of schisandrin B on NRF2/Keap1/PGC-1α signaling pathway in lung tissue of rats with severe pneumonia caused by Klebsiella
- Author:
WANG Yibo
;
JIAO Bin
;
WANG Xiaoqiang
;
CHEN Chixing
;
ZENG Cimei
- Publication Type:Journal Article
- Keywords:
Schisandrin B;
severe pneumonia;
Nrf2/Keap-1/PGC-1α signaling pathway
- From:
China Tropical Medicine
2023;23(12):1313-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Schisandrin B (Sch B) on nuclear transcription factor E2-related factor 2 (Nrf2)/kelch-like epichlorohydrin-associated protein (Keap-1)/ peroxisome proliferation-activated receptor γ coactivator-1α (PGC-1α) signaling pathway in lung tissue of rats with severe pneumonia. Methods A rat model of severe pneumonia was first established and then randomly divided into model group, Sch B low, medium, and high dose groups and positive control group, with 10 rats in each group, and another 10 rats was selected as a blank control group. Sch B low, medium and high dose groups were given intragastrically with 2.50, 5.0, 10.0 mg/kg Sch B for intervention, the positive control group was given 1.04 mg/kg dexamethasone for intervention, and the rest of groups were given equal volume of normal saline, for 14 consecutive days. Aorta blood was taken to detect blood gas index. Lung tissue was isolated, and pathological changes, inflammatory factors and pathway-related protein expression were detected. Results The rats in the control group had normal diet, no abnormal mental state, and clear lung tissue structure. Compared with the control group, the rats in the model group were in a worse state, with symptoms such as unresponsiveness, sluggishness, and shortness of breath, inflammatory infiltration of the lung tissue, edema of the alveolar interstitium, and thickening of the alveolar wall. The PaCO2 value, TNF-α, IL-6 and IL-1β contents, Keap-1 protein expression all increased significantly (P<0.05), the PaO2 and SaO2 levels, Nrf2 and PGC-1ɑ protein expression reduced significantly (P<0.05). Compared with the model group, the adverse symptoms of rats in the Sch B low, medium, and high dose groups alleviated gradually, and the inflammatory infiltration of lung tissue and alveolar interstitial edema reduced gradually, the PaCO2 value, TNF-ɑ, IL-6 and IL-1β contents, and Keap-1 protein expression all decreased sequentially (P<0.05), the PaO2 and SaO2 values, Nrf2 and PGC-1ɑ protein expression levels increased sequentially (P<0.05). The indicators were no significant difference between the Sch B high-dose group and the positive control group (P>0.05). Conclusions Sch B can alleviate the adverse symptoms of severe pneumonia caused by Klebsiella in rats, which may be related to the activation of the NRF2/PGC-1α signaling pathway and the reduction of Keap1 protein expression.
- Full text:202412231714589622013.Effect of schisandrin B on NRF2Keap1PGC-1α signaling pathway in lung tissue of rats with severe pneumonia caused by Klebsiella.pdf
