Killing efficacy of Bacillus thuringiensis israelensis on Aedes albopictus at different temperatures
- Author:
LIU Tingting
;
WANG Ying
;
ZHENG Dan
;
LIU Zhilong
;
LI Xin
;
WANG Jing
;
YU Shasha
- Publication Type:Journal Article
- Keywords:
Bacillus thuringiensis israelensis;
Aedes albopictus;
bioassay;
temperature
- From:
China Tropical Medicine
2023;23(12):1260-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the lethal effect of Bacillus thuringiensis israelensis (Bti) on the 3rd instar larvae of Aedes albopictus and the influence of environmental temperature. Methods Probit analysis bioassay was used to detect comparatively analyze the lethal effect of Bti on third instar larvae of Aedes albopictus under different environmental temperatures. The accumulated mortality rate of larvae after treatment with different Bti concentrations and corresponding P-values and concentration logarithm were used for linear regression analyses to obtain the linear regression formula, and important dose parameters such as LC50 and LC95 were calculated. Result Bti had a significant lethal effect on Aedes albopictus larvae and the drug efficacy was rapid. At a standard 28 ℃, significant larval mortality was observed 12 h after Bti treatment, and the cumulative mortality rate increased with the rise of treatment time and concentration of Bti. Linear regression formulae of the lethal effect of Bti on Aedes albopictus larvae at different time points and temperatures were obtained. The insecticidal effect was ideal between 28-32 ℃ for the same Bti concentration, and the insecticidal effect was relatively weakest in a low temperature environment of 20 ℃; the LC95 at 28 ℃ at each time point was the lowest, with LC95 values of 122.418 IU/L, 109.404 IU/L, 96.474 IU/L, and 92.229 IU/L at 24 h, 48 h, 72 h, and 96 h respectively. Conclusions Bti has a strong lethal effect on the third instar larvae of Aedes albopictus, environmental temperature can influence the insecticidal effect of Bti on Aedes albopictus, raising temperatures within a certain range can enhance the lethal effect; when using Bti in reality, the influence of temperature factors should be fully considered to achieve better insecticidal effect.
- Full text:20241223165043092984.Killing efficacy of Bacillus thuringiensis israelensis on Aedes albopictus at different temperatures.pdf
