Research on Chemical Constituents and Antioxidant Capacity of Opuntia Milpa Alta and Its Fruits Using UPLC-QE-Orbitrap-MS
10.13748/j.cnki.issn1007-7693.20223578
- VernacularTitle:基于UPLC-QE-Orbitrap-MS的米邦塔仙人掌茎果化学成分表征及其抗氧化活性评价
- Author:
Haotian XUE
1
;
Weiyi JIN
2
;
Fangqing ZHANG
3
;
Jingxun WANG
3
;
Yumiao LANG
4
;
Yue SHI
3
Author Information
1. College of Public Health, Hebei University, Baoding 071000, China；The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
2. Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
3. The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
4. College of Public Health, Hebei University, Baoding 071000, China
- Publication Type:Journal Article
- Keywords:
Opuntia Milpa Alta；UPLC-QE-Orbitrap-MS；chemical constituents；antioxidant
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(10):1372-1380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To characterize the chemical constituents of the cladodes and fruits of Opuntia Milpa Alta by using UPLC-QE-Orbitrap MS, and investigate the antioxidant activity, and explore the relationship between the constituents and biological activity.
METHODS
The UPLC HSS T3 C18 column(2.1 mm×10 mm, 1.8 μm) was used. Acetonitrile and water with 0.1% formic acid was used for gradient elution at a 0.2 mL·min–1 flow rateas. Heated electrospray ion source was used for primary and secondary mass spectrometry data acquisition in positive and negative ion modes, respectively. Database and literature reports were used to characterize the chemical constituents of the cladodes and fruits of Opuntia Mipa Alta. DPPH radical, hydroxyl radical(·OH) and superoxide anion radical(·
) scavenging ability were used to investigate the antioxidant activities of the cladodes and fruits of Opuntia Milpa Alta.
RESULTS
A total of 39 compounds were characterized from the cladodes and fruits of Opuntia Milpa Alta, including 22 phenolic compounds, 13 flavonoids and 4 other components. Both cladodes and fruits of Opuntia Milpa Alta had certain antioxidant capacity. The fruits of Opuntia Milpa Alta had better scavenging ability of DPPH than the cladodes, while the cladodes had a slightly stronger scavenging ability of ·OH than the fruits. The scavenging ability of ·
between cladode peels and fruit peels was not significantly different, and the juice had the strongest scavenging ability of ·
. The antioxidant activity of Opuntia Milpa Alta was closely related to its rich phenolic acids.
CONCLUSION
In this study, a rapid, accurate and reliable method is established to identify the chemical constituents of the cladodes and fruits of Opuntia Milpa Alta, which provides scientific basis for the comprehensive development and utilization of Opuntia Milpa Alta.
