Analysis of Specific Chromatogram of Classical Formula Qianghuo Shengshi Tang Reference Samples
- VernacularTitle:经典名方羌活胜湿汤基准样品的特征图谱分析
- Author:
Wenya GAO
1
;
He XU
2
;
Mingli LI
1
;
Haiyu ZHAO
1
;
Yanyan ZHOU
1
;
Hongjie WANG
1
;
Jian YANG
1
;
Xiaolu WEI
1
;
Zhikun FAN
2
;
Nan SI
1
;
Baolin BIAN
1
Author Information
- Publication Type:Journal Article
- Keywords: famous classical formulas；Qianghuo Shengshi Tang；reference sample；specific chromatogram
- From: Chinese Journal of Modern Applied Pharmacy 2024;41(10):1350-1356
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE :To establish the specific chromatogram of Qianghuo Shengshi Tang(QHSS) reference sample, clarify the key quality attributes of QHSS, providing reference for the quality evaluation of QHSS reference sample. METHODS The SilGreen C18 column(4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted acetonitrile and 0.2% formic acid aqueous solution. The detection wavelength was 328 nm. Established an HPLC characteristic spectrum analysis method for the reference sample of QHSS. A variety of chromatographic columns and different instruments were applied to investigate the adaptability of the system. HPLC-LTQ-Orbitrap MS was used to identify the specific peaks of the QHSS reference samples in positive ion mode. RESULTS There were 14 peaks in the specific chromatogram, which belonged to Notopterygii Rhizoma Et Radix, Angelicae Pubescentis Radix, Ligustici Rhizoma Et Radix, Chuanxiong Rhizome, Viticis Fructus, respectively. Ferulic acid(peak 3) was reference peak. A total of 22 compounds were identified by mass spectrometry, including coumarin and flavonoids. CONCLUSION The established specific chromatogram method of QHSS is simple, stable and reproducible. The material basis of QHSS reference sample is basically determined, providing a reference for the development and quality control of QHSS.
