Rapid Analysis of the Chemical Composition of Zhangbang Nine Steaming Nine Sun-dying Rehmanniae Radix Based on UPLC-Q-TOF-MS Technique and Study on the Function of Solid Excipiemts in Alleviating the "Nourishing and Spleen-impairing" in Rehmanniae Radix
10.13748/j.cnki.issn1007-7693.20223933
- VernacularTitle:基于UPLC-Q-TOF-MS技术快速分析樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能研究
- Author:
Xudong ZHU
1
;
Wei YAO
2
;
Feixia YAN
2
;
Dong HUANG
3
;
Song WANG
2
;
Na ZHU
1
;
Mingming YUAN
3
;
Liping HUANG
2
;
Yi WU
3
Author Information
1. Jiangxi Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Jiangxi Province Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China；Jiangxi University of Chinese Medicine, Nanchang 330004, China
2. Jiangxi University of Chinese Medicine, Nanchang 330004, China
3. Jiangxi Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Jiangxi Province Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS；nine steaming nine sun-dying；Rehmanniae Radix ； nourishing and spleen-impairing；chemical composition； gastric emptying；intestinal propulsion
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(10):1332-1342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To study the chemical composition and the function of solid excipients in relieving the "nourishing and spleen-impairing" effect of Zhangbang nine steaming nine sun-dying Rehmanniae Radix(RR) by using UPLC-Q-TOF-MS technique.
METHODS
UPLC-Q-TOF-MS was used to collect the data of Zhangbang nine steaming nine sun-dying RR, and UNIFI platform and self-built database were used for the rapid qualitative analysis and identification of the chemical components of Zhangbang nine steaming nine sun-dying RR. The effect of solid excipients on the function of RR "nourishing and spleen-impairing" was investigated by establishing a mouse model of low gastric motility and measuring its gastric residual rate and small intestine propulsion rate.
RESULTS
The UPLC-Q-TOF-MS and UNFI platforms identified 76 chemical components in nine steaming nine sun-dying RR, including 9 iridoid glycosides, 11 phenylethyl glycosides, 31 flavonoids, 6 saccharides and 19 other compounds; the results of gastric emptying and small intestinal propulsion experiments on mice showed that Zhangbang nine steaming nine sun-dying RR had gastric emptying and intestinal propulsion effects.
CONCLUSION
This method can be used for the rapid identification and analysis of nine steaming nine sun-dying RR, which has solid excipients Citri Reticulatae Pericarpium and Amomi Fructus to alleviate the "nourishing and spleen-impairing" function of RR. This study can provide a reference for quality control and elucidation of the material basis of the medicinal effects of specialty beverage of Zhangbang nine steaming nine sun-dying RR.
