Effect of Irradiation Sterilization on the Quality of Rubus Chingii Hu
10.13748/j.cnki.issn1007-7693.20230914
- VernacularTitle:辐照灭菌对覆盆子品质的影响
- Author:
Ziqian CAI
1
;
Wenting ZHANG
2
;
Ting DONG
2
;
Xinmei CHENG
3
;
Lifeng HANG
3
;
Sheng DING
3
;
Zengxi GUO
2
;
Cuifen FANG
2
;
Bilian CHEN
2
Author Information
1. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310052, China
2. Zhejiang Institute for Food and Drug Control, Hangzhou 310053, China；NMPA Key Labortory for Quality Evaluation of Traditional Chinese Medicine(Traditional Patent Chinese Medicine), Hangzhou 310053, China
3. Hangzhou Huqingyutang Pharmaceutical Co., Ltd., Hangzhou 310016, China
- Publication Type:Journal Article
- Keywords:
Rubus chingii Hu； irradiation sterilization；HPLC；content determination；fingerprint； cluster analysis；principal component analysis
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(9):1222-1228
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To study the effect of 60Co-γ irradiation on the sterilization effect and main components of Rubus chingii Hu.
METHODS
Irradiated Rubus chingii Hu by 0, 6, 10, 15, 30 kGy doses of 60Co-γ, used the microbial count method to determine the microbial level of Rubus chingii Hu before and after irradiation. Analyzed the components of Rubus chingii Hu by high resolution mass spectrometry, investigated the effects of irradiation on the quality of Rubus chingii Hu by comparing the components of Rubus chingii Hu samples before and after irradiation, analyzing the quantitative results of ellagic acid and kaempferol 3-O-yunxiangoside, and evaluating the similarity of fingerprints.
RESULTS
The results of microbial examination of Rubus chingii Hu after different doses of irradiation all met the requirements, cluster analysis and principal component analysis of 20 components showed no significant difference. And there was no significant difference in the contents of ellagic acid and kaempferol 3-O-glucoside before and after irradiation. The similarity of fingerprints before and after irradiation was between 0.995 and 1.000.
CONCLUSION
Irradiation can effectively control the microbial level in Rubus chingii Hu, and there is no significant effect on the chemical composition of Rubus chingii Hu, the results provide a basis for the application of irradiation in the sterilization process of Rubus chingii Hu.
