Effect of Jinshui Liujun Decoction on Pulmonary Water Metabolism and TNF-α/NF-κB Signaling Pathway in Asthmatic Mice
10.13748/j.cnki.issn1007-7693.20231245
- VernacularTitle:金水六君煎对哮喘小鼠肺水代谢及TNF-α/NF-κB信号通路的影响
- Author:
Ying YANG
1
,
2
;
Jianhong XI
3
;
Zhongliang TANG
1
,
2
;
Ying XIA
1
,
2
;
Ying LI
1
,
2
;
Xiaoli CHENG
3
;
Zhiwang WANG
3
Author Information
1. The Third People'
2. s Hospital of Gansu Province, Lanzhou 730000, China
3. Gansu University of Chinese Medicine, Lanzhou 730000, China
- Publication Type:Journal Article
- Keywords:
Jinshui Liujun decoctions；asthma； pulmonary water metabolism;
aquaporins；TNF-α/NF-κB signal pathway
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(9):1168-1172
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To study effects of Jinshui Liujun decoction(JLD) on pulmonary water metabolism and TNF-α/NF-κB signaling pathway in asthmatic mice.
METHODS
After 1 week of acclimatization, 10 mice were randomly selected by body weight as a blank control group, and the rest of the mice were sensitized by intraperitoneal and subcutaneous injections of ovalbumin sensitizing solution on the 1st, 8th, and 15th day of the experiment. On the 22nd day, they were randomly divided into model control group, positive control group and JLD low, medium and high dose(4.1, 8.2, 16.4 g·kg−1) group, and replicated the asthma mouse model by inhaling nebulized excitation solution(1% ovalbumin) once every other day for 2 consecutive weeks, and at the same time, were given atomized excitation solution(1% ovalbumin) every other day for two weeks to replicate the asthmatic mouse model, and were given the test drug by gavage daily. The effects of JLD on the behavior, lung wet-dry weight ratio, AQP1, AQP5, TNF-α, NF-κB, and NF-κB mRNA expression in asthmatic mice were observed.
RESULTS
JLD reduced the number of animals with asthma attacks, alleviated asthma symptoms, decreased the lung index and lung wet-to-dry weight ratio, increased the expression level of AQP1 and AQP5 in lung tissues, reduced the content of TNF-α in bronchoalveolar lavage fluid and lung tissues, and down-regulated the expression level of NF-κB and its genes in lung tissues(P<0.05 or P<0.01).
CONCLUSION
JLD has an anti-asthma effect and its mechanism of action is partially due to its inhibition of the TNF-α/NF-κB signaling pathway, upregulation of AQP1 and AQP5, and improvement of pulmonary water metabolism.
