Changes of gut microbiota before and after treatment of technetium [ 99 Tc] methylenediphosphonate injection in rheumatoid arthritis
10.19405/j.cnki.issn1000-1492.2022.02.028
- Author:
Changming Chen
1
;
Zhaowei Huang
1
;
Ping Zeng
1
;
Xueming Yao
1
;
Can Liu
1
;
Ying Wang
1
;
Wukai Ma
1
Author Information
1. Dept ofRheumatology and Immunology, The Second Afiliated Hospital of Guizhou University of Traditional Chinese Medicine , Guiyang 550001
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
gut microbiota;
99 Tc⁃MDP;
16S rRNA
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):310-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the changes of intestinal flora in patients with rheumatoid arthritis (RA) before and after treatment with technetium[ 99 Tc] methylenediphosphonate injection ( 99 Tc⁃MDP) .
Methods :Thirty⁃two patients with RA were collected the fresh fecal specimens before and after they treated with 99 Tc⁃MDP. The genome DNA of fecal specimens was extracted and the V3 ⁃V4 regions of 16S rRNA were sequenced by Illumina Miseq platform. The sequencing results were analyzed by bioinformatics methodology.
Results : The Chao , observed species , Shannon and Simpson index between the two groups in Alpha diversity analysis had no statistical difference (P > 0. 05 ) , but the value in post⁃treatment group were increased compared with the pre⁃treatment group. Compared with the pre⁃treatment of RA , there was no statistical difference in the higher proportion relative abundance on Phylum level of the intestinal microbes of RA patients , and the relative abundance of Tenericutes decreased ( P < 0. 05 ) . At the genus level , the relative abundance of Ruminococcaceae , Butyricicoccus , Christensenellaceae_R ⁃7_group , Holdemania decreased after treatment ( P < 0. 05 ) . And the relative abundances of Pasteurella , Lachnoanaerobaculum and Stomatobaculum increased (P < 0. 05) .
Conclusion :The abundance and species of gut microbiota in RA patients have some changes after treatment with 99Tc⁃MDP , and the diversity increases after treatment.
- Full text:2024121914554728217锝[~(99)Tc]亚甲基...节炎患者前后的肠道菌群变化_陈昌明.pdf
