Simultaneous Determination of 14 Components in Qingfei Yihuo Tablets by HPLC
10.13748/j.cnki.issn1007-7693.20224248
- VernacularTitle:HPLC同时测定清肺抑火片中14种成分的含量
- Author:
Wei'er XU
1
;
Xiuchun FAN
1
;
Tingshun LUO
1
;
Mingjin MIAO
1
;
Kun DONG
1
;
Huaijing YANG
1
Author Information
1. Dali Institute for Food and Drug Control, Dali 671000, China
- Publication Type:Journal Article
- Keywords:
Qingfei Yihuo tablets； HPLC-DAD； content determination； quality control
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(8):1084-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish a high-performance liquid chromatography method for the simultaneous determination of 14 components including geniposide, mangiferin, baicalin, berberine hydrochloride, wogonoside, baicalein, aloe-emodin, rhein, wogonin, emodin, praeruptorin A, chrysophanol, physcion and praeruptorin B in Qingfei Yihuo tablets.
METHODS
Titank C18 (250 mm×4.6 mm, 5 μm) column was used; 0.1% phosphoric acid solution(A)-acetonitrile(B) was used as the mobile phase with gradient elution; detection wavelengths: geniposide at 238 nm, aloe-emodin, rhein, emodin, chrysophanol, physcion and mangiferin at 254 nm, berberine hydrochloride at 265 nm, baicalin, wogonoside, baicalein, and wogonin at 280 nm, praeruptorin A and praeruptorin B at 321 nm. The flow rate was 1.0 mL·min−1 and the column temperature was 35 ℃; the injection volume was 10 μL.
RESULTS
The linear ranges of geniposide, mangiferin, baicalin, berberine hydrochloride, wogonoside, baicalein,aloe-emodin, rhein, wogonin, emodin, praeruptorin A, chrysophanol, physcion and, praeruptorin B were 4.96−223.17, 0.84−42.22, 18.76−938.16, 4.46−223.17, 4.86−243.10, 1.59−79.32, 0.76−38.17, 1.03−51.49, 1.59−79.40, 1.21−60.72, 1.80−90.06, 0.91−45.48, 1.04−51.83 and 0.86−43.23 μg·mL−1, with r all ≥ 0.999 9. The RSDs of instrument precision, stability and reproducibility tests were <3%, and the average recoveries in sample(n=6) were >90%, with the RSDs <3%.
CONCLUSION
The method is simple and reproducible and can provide a scientific basis for improving the quality standard of Qingfei Yihuo tablets.
