Comparison of the Quality of Sheep Bile from Different Regions Based on UHPLC-ELSD Fingerprint and Multi-component Content Determination Combined with Antioxidant Activity
10.13748/j.cnki.issn1007-7693.20232863
- VernacularTitle:基于UHPLC-ELSD指纹图谱及多成分含量测定比较不同产地羊胆汁质量及抗氧化活性
- Author:
Xuxiang ZHOU
1
;
Qianqian ZHU
1
;
Dandan ZHANG
1
;
Xinyao LUO
1
;
Dan LIU
1
;
Min ZHANG
2
;
Xiaochuan YE
1
Author Information
1. Hubei University of Traditional Chinese Medicine Hubei Provincial Key Laboratory of Traditional Chinese Medicine Resources and Traditional Chinese Medicine Chemistry at School of Pharmacy
2. Hubei University of Traditional Chinese Medicine Traditional Chinese Medicine Experimental Center, Wuhan 430065, China
- Publication Type:Journal Article
- Keywords:
sheep bile ； fingerprint；content determination； antioxidant activity；entropy weight TOPSIS ；grey correlation analysis； quality control
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(8):1066-1074
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To establish UHPLC-ELSD fingerprint and multi-component content determination methods, compare the differences in sheep bile from different regions, and conduct antioxidant activity research to provide a basis for the in-depth development and utilization of sheep bile.
METHODS
Used UHPLC-ELSD method to establish 21 batches of bile fingerprints of sheep from different origins and conduct similarity analysis. Measured the content of 6 components, DPPH and ABTS free radical scavenging ability, iron ion reduction ability, and conducted entropy weighted TOPSIS and grey correlation analysis.
RESULTS
A total of 11 common peaks were identified in the fingerprint spectra of 21 batches of sheep bile. Through comparison with the control sample, 6 components were identified, including taurocholic acid(TCA), glycocholic acid(GCA), taurochenodeoxycholic acid(TCDCA), tauroursodeoxycholic acid(TDCA), glycodeoxycholic acid(GDCA), and cholic acid(CA). Except for 4 batches of samples, the similarity of the fingerprint spectra was greater than 0.90. The total content range of 6 components in the freeze-dried powder of 21 batches of sheep bile was 55.34% to 86.08%. The highest content of taurocholic acid ranged from 34.74% to 60.86%, indicating significant differences in the content of the six components in samples from different regions. Sheep bile from different regions had antioxidant activity, and there were also certain differences. The results of entropy weighted TOPSIS analysis using six component contents as variables showed that the top ten scoring groups were S2, S18, S16, S9, S8, S21, S1, S10, S20, and S15, indicating good quality and slightly better bile quality from sheep in the northern region. The grey correlation analysis results between the content of 6 components and 3 antioxidant indicators showed that all 6 components were correlated with each antioxidant indicator, and TCA, TDCA, and TCDCA had the highest correlation, which might be important components for sheep bile to exert antioxidant effects.
CONCLUSION
The use of entropy weighted TOPSIS and grey correlation analysis methods can effectively analyze the quality differences and antioxidant active components of sheep bile from different regions, providing scientific basis for its quality evaluation.
