Effects of Sanhuang Gel on p38MAPK Signaling Pathway and Its Key Molecules in Rat Auricle Acne Complex Model
10.13748/j.cnki.issn1007-7693.20221129
- VernacularTitle:三黄凝胶对大鼠耳廓复合痤疮模型p38MAPK信号通路及其关键分子的影响
- Author:
Dan LI
1
;
Xueyang DU
1
;
Sinong WANG
1
Author Information
1. Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
- Publication Type:Journal Article
- Keywords:
Sanhuang gel ； acne；p38MAPK signaling pathway；MCP-1；HMGB1 ；α-SMA
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(8):1035-1046
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To observe the anti-inflammatory mechanism of Sanhuang gel on rat model of auricle acne and its possible mechanism with p38MAPK signaling pathway as the entry point.
METHODS
Sixty-two male Wistar rats were divided into blank group and modelling group. The modeling group replicated the experimental animal model of acne(application of 100% oleic acid+subcutaneous injection of Propionibacterium acnes), and 2 rats were randomly selected for HE staining after 21 days. The remaining rats were randomly divided into model group, positive control group(Vitamin A cream 1.48 g·d−1 ), Sanhuang gel high, medium and low-dose group(1.1, 0.568, 0.3 g·d−1 ). They were administered for 8 weeks, and observed on the 7, 14 and 28 day of treatment. The right auricle tissues were collected, and the histopathological changes were observed by HE stain. The mRNA expressions of p38MAPK, MKK3 and MKK6 were detected by RT-PCR. The protein expressions of MCP-1, HMGB1 and α-SMA in auricle tissues were detected by IHC and Western blotting.
RESULTS
Compared with blank group, the epidermis of auricular tissue in model group was severely keratinized, inflammatory cell infiltration was obvious, mRNA levels of p38MAPK, MKK3, MKK6 and protein expression levels of MCP-1, HMGB1 and α-SMA were increased(P<0.05). After drug therapy intervention, auricle apparent score at different time points in each treatment group was improved to varying degrees. The mRNA levels of p38MAPK, MKK3, MKK6 and protein levels of MCP-1, HMGB1 and α-SMA in Sanhuang gel high-dose and medium-dose groups and positive control group were decreased(P<0.05), while in Sanhuang gel low-dose group, the expressions of MKK3 mRNA and MCP-1, HMGB1 protein were decreased(P<0.05), the expression of p38MAPK, MKK6 mRNA and α-SMA protein decreased not significantly.
CONCLUSION
The mechanism of the effect of Sanhuang gel on the inflammatory response of rat compound acne animal model may be related to its intervention of p38MAPK protein feedback regulation signaling pathway and inhibition of MCP-1, HMGB1 and α-SMA expression.
