Determination of Chemical Components and Blood Entering Components of Weite’an Tablets Hospital Preparations Based on UHPLC-Q-TOF-MS
10.13748/j.cnki.issn1007-7693.20224184
- VernacularTitle:UHPLC-Q-TOF-MS检测医院制剂胃特安片主要化学成分及入血成分
- Author:
Mi PENG
1
,
2
,
3
;
Lan XU
3
,
4
;
Zengzhu ZHANG
3
,
4
;
Gang LI
3
,
4
;
Kuangyi LIU
5
Author Information
1. Nanchang People&rsquo
2. s Hospital (Nanchang Third Hospital), Nanchang 330000, China；The 908th Hospital of the Joint Logistics Support Force of the Chinese People&rsquo
3. s Liberation Army, Nanchang 330000, China
4. The 908th Hospital of the Joint Logistics Support Force of the Chinese People&rsquo
5. The First Hospital of Nanchang, Nanchang 330000, China
- Publication Type:Journal Article
- Keywords:
Weite’an tablets ；UHPLC-Q-TOF-MS ；chemical composition ；hospital preparation ； blood components
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(7):946-956
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To analyze and identify the chemical components of Weite’an tablet which is a hospital preparation by UHPLC-Q-TOF-MS, and to find the drug migration components in rat plasma.
METHODS
In 0.05% formic acid water acetonitrile mobile phase system, after chromatographic separation of the Acquire UPLC BEH C18 column, Q-TOF positive and negative ion scanning was performed, and data processing was performed in SWATH collection mode. At the same time, quantitative analysis of various components in plasma samples was conducted through MRMHR data processing function during qualitative identification.
RESULTS
A total of 52 chemical components were isolated and identified in the experiment, including 22 flavonoids, 6 phenolic acids, 5 terpenoids, 3 coumarins, 2 alkaloids, amino acids and other compounds. Eight components were introduced into the blood: 6-hydroxy-7-methoxycoumarin, hesperidin, tangerine, stachydrine, berberine, scutellarin and quercetin.
CONCLUSION
This method is accurate and stable, and is suitable for the analysis and identification of chemical components in Weite’an tablets. The experimental results lay a solid foundation for clinical rational drug use, follow-up drug tissue distribution and basic research of effectors.
